Histologistics is proud to offer control slides! Do you need quality controls for your special stains?
We can now provide high quality positive control tissues. Controls slides normally come with positive and negative controls on every slide but can be customized to meet your needs. All slide sets are cut and sent after payment has been made. All of our slides are guaranteed, and will do whatever it takes if you are dissatisfied. We offer routine prices that ship within 2 weeks of ordering and rush services that can be shipped within 2 days of order. All first time customers will receive slides at $2.00 each for their first order. Choose either the Histopathology or immunohistochemistry control page for tissues available, then call or e-mail us today.
Background: The tissue and control are on the same slide, and the control is staying on but tissue is falling off.
There’s a lot of could be answers, that you might have to try before finding the answer. So here is a list, answers by histologists around the world.
Try not baking the control tissue just let them air dry, the humidity in the slide drying oven might be messing with the super frost plus slides. It’s just a coating on the slide of some sort, after the first dip they don’t work again like the first time, so just air dry the controls and bake after patient tissue is picked up.
Water is always a potential issue. Tissue can be finicky though. Have you tried poly-L-lysine coated slides? They do help is some occasions.
If you are drying your slides in the oven, check the temp and
Try going longer and tap off excess water before drying.
If the sections are coming off the slides entirely, or does it have a “chewed up” appearance? If the sections are coming completely off, I would agree with the others that water is the issue.
Also, make sure you are cutting no thicker than 4 microns, and let the sections really flatten out on the water bath for a minute.
Bake them well.
If the sections have more of a chewed up appearance, then it’s probably a combination of factors: fatty tissue, not well fixed, strong antigen retrieval.
After baking, “post fix” some of the IHC slides in formalin for about 15 minutes. Rinse in tap water then put on stainer as usual. This seems to help when certain antibodies like to chew up the tissue.
Another thing to try is different slides. There issues with bad batches/lots of slides – even the Fisher Superfrost plus.
Make sure the PM is done on the machine quarterly.
We have an abundance of human helicobacter pylori (HP) tissue. This is great for immunohistochemistry control tissue. While trying out a potential tissue supplier, we order way to much tissue and now have no room for it all. To rectify this we are practically giving it away. All slides for $2.00 each, minimum 50 slides please. All orders are cut to order and includes the first and last slides stained with H&E. Slide boxes are extra but you can save money by sending us your own slide box. Please help us reduce our inventory!