Posted on 3 Comments

Masson’s Trichrome Green Protocol

Masson’s Trichrome Green Protocol

1. De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
2. De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
3. Clear slides in 100% alcohol———————– 2 minutes
4. Clear slides in 100% alcohol———————– 2 minutes
5. Hydrate slides in 95% alcohol———————- 2 minutes
6. Hydrate slides in running water——————– 5 minutes
7. Bouin’s Fixative (60 Celsius)———————- 30 minutes (re-use)
8. Wash in running water—————————— 5 minutes
9. Weigerts——————————————- 10 minutes (dump)
10. Wash in running water—————————— 5 minutes
11. Biebrich scarlet acid fuchsin———————- 5 minutes (re-use)
12. Rinse quickly————————————– 30-60 seconds
13. Phostung-Phosmoly acid (PTA-PMA)——————- 5 minutes (re-use)
14. No rinse
15. 2.5% Fast green———————————— 5 minutes (re-use)
16. Rinse quickly ————————————- 30 seconds
17. 1% Glacial acetic acid—————————– 2 minutes (re-use)
18. Dehydrate in 95% alcohol————————— 30 seconds
19. Dehydrate in 100% alcohol————————– 1 minute
20. Dehydrate in 100% alcohol————————– 2 minutes
21. Dehydrate in 100% alcohol————————– 2 minutes
22. Clear in xylene (3)——————————– 2 minutes (re-use)
23. Clear in xylene (4)——————————– 5 minutes (re-use)

Results:
Nuclei——————— Black to purple
Cytoplasm- —————- Shades of pink, red
Erythrocytes and myelin—- Scarlet
Collagen fibers———— Strong bluish-green
Mucus———————- Lighter green

Posted on Leave a comment

Histology service

histology service lipid

We have an extensive array of histology knowledge, from years of working in clinical, research and academia histology labs.  Our techniques have been modified to give you excellent results and turnaround times.  The desire to help your causes, through research advance faster through the end stage of a histology service drives our success. We understand that your research is the most important thing in the world and our job is to make your life easy by doing everything right on our end within a reasonable time.

Do to this, we have built our lab for efficiency and quality.  All specimens come in and leave in a circular fashion. This eliminates redundancy and waste by creating checks and balances that make a lean lab.  We believe, it’s our job to make you look good.

Come see what we can do for you.

Posted on

Histology Lab

  Histologistics is a full service histology lab. We provide excellent quality histology to clinical facilities, veterinarians and researchers . We can help you with anything.  Please contact us with your histology needs.