After staining a series of small intestine slides with Alcian blue pH2.5 for controls I noticed little black dots in the tissue. Upon closer look, I believe those black things are fungi. The alcian blue stain still looks good and slides can be used for that stain but I’ve never seen fungi like this before.
We will be posting all of our special stain protocols along with an example of that stain. This will give a database like website from which to search.
We did this as a test to see different versions of luxol fast blue (LFB). LFB is a stain for the myelin sheath or myelinated axons in brain and spinal cord. We tested on rat brain. There are 4 variations. They are; LFB / Cresyl violet (CR), LFB /H&E, LFB / Periodic acid schiff (PAS) and LFB / nuclear fast red (NFR). The H&E and NFR versions are for background staining only. The PAS could show glycogen or fungus present and the CR shows nissl bodies.