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Sm Intestine / Alcian Blue / Fungus

After staining a series of small intestine slides  with Alcian blue pH2.5 for controls I noticed little black dots in the tissue.  Upon closer look, I believe those black things are fungi.  The alcian blue stain still looks good and slides can be used for that stain but I’ve never seen fungi like this before.

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Luxol Fast Blue Stain Variations

We did this as a test to see different versions of luxol fast blue (LFB). LFB is a stain for the myelin sheath or myelinated axons in brain and spinal cord.  We tested on rat brain.  There are 4 variations.  They are; LFB / Cresyl violet (CR), LFB /H&E, LFB / Periodic acid schiff (PAS) and LFB / nuclear fast red (NFR).   The H&E and NFR versions are for background staining only.  The PAS could show glycogen or fungus present and the CR shows nissl bodies.

Rat brain LFB / CV 20x
Rat brain LFB / CV 20x
Rat brain LFB / H&E 20x
Rat brain LFB / H&E 20x
Rat brain LFB / NFR 20x
Rat brain LFB / NFR 20x
Rat brain LFB / PAS 20x
Rat brain LFB / PAS 20x