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Small Business Histology

Come experience our extremely skilled and experienced technical team to show what your experiment results could look like.    Try us today.

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An Expert Histology Service

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Hematoxylin & Eosin (H&E) Protocol

Safety equipment: Work under a hood with lab coat, gloves, and glasses.

  1. De-paraffin slides in xylene (1) ——————  2 minutes (re-use)
  2. De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
  3. Clear slides in 100% alcohol———————- 2 minutes
  4. Clear slides in 100% alcohol———————- 2 minutes
  5. Hydrate slides in 95% alcohol——————– 2 minutes
  6. Hydrate slides in running water—————–  5 minutes
  7. Stain slides in Harris Hematoxylin————–  10 minutes (re-use)
  8. Rinse slides in running water———————  2 minutes
  9. Differentiate in acid alcohol———————-   1 quick dip (re-use)
  10. Rinse in water————————————–    3 dips
  11. Dip in ammonia water—————————-    15-30 seconds (re-use)
  12. Wash in running tap water———————–    10 minutes
  13. Place in 95% alcohol——————————    2 minutes
  14. Stain in eosin—————————————    1 minute (re-use)
  15. Dehydrate in 95% alcohol————————   1 minute
  16. Dehydrate in 100% alcohol———————-   1 minute
  17. Dehydrate in 100% alcohol———————-   2 minutes
  18. Dehydrate in 100% alcohol———————-   2 minutes
  19. Clear in xylene (3)———————————   2 minutes (re-use)
  20. Clear in xylene (4)———————————   5 minutes (re-use)

Results: Nuclei –Blue   Cytoplasm – Pink     Blood – Red

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Histology service

histology service lipid

We have an extensive array of histology knowledge, from years of working in clinical, research and academia histology labs.  Our techniques have been modified to give you excellent results and turnaround times.  The desire to help your causes, through research advance faster through the end stage of a histology service drives our success. We understand that your research is the most important thing in the world and our job is to make your life easy by doing everything right on our end within a reasonable time.

Do to this, we have built our lab for efficiency and quality.  All specimens come in and leave in a circular fashion. This eliminates redundancy and waste by creating checks and balances that make a lean lab.  We believe, it’s our job to make you look good.

Come see what we can do for you.

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Histology Lab

  Histologistics is a full service histology lab. We provide excellent quality histology to clinical facilities, veterinarians and researchers . We can help you with anything.  Please contact us with your histology needs.