We have been working hard to validate many new antibodies for research use. Vimentin is now available for use on Human, mouse and rat FFPE tissues.
According to the data sheet Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.
Pathology of colon cancer as seen through the eyes of a histologist. How many different ways are there to visualize cancer? The more the better!
Hematoxylin & Eosin (our recipe)
Human colon adenocarcinoma H&E
HMGB1 Antibody – According to the The Human Protein Atlas HMGB1 protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration.
Human colon adenocarcinoma HMGB1
CD8 Antibody in the Human Protein Atlas ( CD8A) describes it as a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.
Human colon adenocarcinoma CD8
Indoleamine Antibody – This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity (IDO1).
Human Colon adenocarcinoma IDO1
Human Colon adenocarcinoma IDO1
Perforin Antibody – This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis (Perforin).
Colon Adenocarcinoma Perforin Antibody
Note: All tissues were bought, cut and stained at Histologistics Inc as positive control slides. These are not part of any study.
For us in histology, normal tissue identification is typically an easy process. As histologists, we were once required to identify all normal tissues by H&E under the microscope.
For abnormal tissue however, an H&E stained slide can be very difficult to identify tissue type and diagnosis. That’s why when a pathologist orders multiple stains on one tissue, they are trying to get a larger picture to understand its etiology.
To help in understanding, here are 3 stains on the same tissue, H&E, SMA, & CD8 at 4x & 10x. This is human tissue. Can you identify this tissue and its diagnosis?
Histologistics is a full service histology lab. We provide excellent quality histology to clinical facilities, veterinarians and researchers . We can help you with anything. Please contact us with your histology needs.