Posted on 8 Comments

Human Skin Tissue Control Slides On Sale $1.37 Per Slide

This is an incredible tissue control sale that will only last 30 days.  All slides are cut to order and can be customized to meet your needs.  We can place negative control tissue right next to the positive tissue and orient to your specifications. Orders will be taken be email or call us at the number below at a first come first serve basis.  No order is considered too small or too large, if we still have the tissue you will get it.

Skin is a very versatile tissue that can be used for a variety of special stains, immunohistochemistry and immunofluorescent stains.  It can be used for special stains such as;

  1. Verhoeff Van Gieson (elastic)
  2. T-blue
  3. Trichrome
  4. Movats
  5. Orcein
  6. PTAH
  7. Picrosirius Red
  8. And others

Controls for immunohistochemistry and immunofluorescent stains include;

  1. CAM 5.2
  2. EMA
  3. Pan-keratin
  4. MNF-116
  5. smooth muscle actin 1A4
  6. S100
  7. Melanin / Mart-1
  8. CD1a
  9. CD117 / C-kit
  10. Langerin
  11. 8-OHdG
  12. Ber-EP4 (epithelial antigen)
  13. Bim
  14. Caspase 3
  15. Cdk4
  16. CDw49f (integrin-alpha6)
  17. Claudin 1
  18. Collagen III, IV
  19. CK 6, 8, 10, 14, 16,17, 18
  20. And many others

Human skin VVG40x

 

Human Skin T-Blue2 20x Human skin MT blue Skin sirius red polar

Skin Fluor human skin H&E 40x

 

Histologistics Inc.

Hans B Snyder
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans@histologistics.com

Histologistics Inc. Privacy Policy &Terms and Conditions 2013-2014

Posted on 2 Comments

Special Stain Solution Protocols Page

Hello,  We have starting adding our solution protocols on a new page.  These are all the protocols that we use now. A professional histology service.

http://histologistics.com/histology/special-stain-solution-protocols/

An Expert Histology Service

Posted on Leave a comment

Orcein for Elastic Fibers Stain Protocol

This elastic stain is far easier than Verhoeff Van Gieson stain but the chemical can be more unusual to find.

Run a control slide.             Control Tissue – Aorta, Skin

Safety equipment: Work under a hood with lab coat, gloves, and glasses.

  1. De-paraffin slides in xylene (1) —————– 2 minutes (re-use)
  2. De-paraffin slides in xylene (2)—————— 2 minutes (re-use)
  3. Clear slides in 100% alcohol——————— 2 minutes
  4. Clear slides in 100% alcohol——————— 2 minutes
  5. Hydrate slides in 95% alcohol——————- 2 minutes
  6. Hydrate slides in running water—————– 5 minutes
  7. Stain in Orcein ————————————– 30 minutes (re-use)
  8. 70% alcohol —————————————– 10 minutes
  9. 70% alcohol —————————————– 10 minutes
  10. 10.  70% alcohol —————————————- 10 minutes
  11. 11.  Rinse in running water ————————– 1 minute
  12. 12.  Stain in Harris Hematoxylin ——————– 1 minute (re-use)
  13. 13.  Rinse in running water ————————– 1 minute
  14. 14.  Acid alcohol —————————————- 1 dip (re-use)
  15. 15.  Rinse in running water ————————– 5 minutes
  16. 16.  Dehydrate in 95% alcohol———————- 30 seconds
  17. 17.   Dehydrate in 100% alcohol——————- 1 minute
  18. 18.   Dehydrate in 100% alcohol——————- 2 minutes
  19. 19.   Dehydrate in 100% alcohol——————- 2 minutes
  20. 20.   Clear in xylene (3)—————————— 2 minutes (re-use)
  21. 21.   Clear in xylene (4)—————————— 5 minutes (re-use)

Results:         Elastic Fibers – Dark Pink

 

 

An Expert Histology Service