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We did this as a test to see different versions of luxol fast blue (LFB). LFB is a stain for the myelin sheath or myelinated axons in brain and spinal cord. We tested on rat brain. There are 4 variations. They are; LFB / Cresyl violet (CR), LFB /H&E, LFB / Periodic acid schiff (PAS) and LFB / nuclear fast red (NFR). The H&E and NFR versions are for background staining only. The PAS could show glycogen or fungus present and the CR shows nissl bodies.