Posted on 2 Comments

Positive Control Slides (Amazing Prices)

Take a look at these high quality control slides.  We have human and animal controls with prices that cannot be beat.  All controls are considered custom orders and will be cut after orders have been placed.  We have an abundant supply of animal tissue controls and a limited supply of human controls.  They are all at the lowest prices guaranteed.

Elastic

Posted on Leave a comment

Mallory PTAH Stain Protocol For Muscle Striations

This is an old stain not used much anymore for diagnosis.  It has been replaced by immunohistochemisty.

Safety equipment: Work under a hood with lab coat, gloves, and glasses.

  1. De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
  2. De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
  3. Clear slides in 100% alcohol———————- 2 minutes
  4. Clear slides in 100% alcohol———————- 2 minutes
  5. Hydrate slides in 95% alcohol——————– 2 minutes
  6. Hydrate slides in running water—————— 5 minutes
  7. If sections are formalin fixed, mordant in Zenker fixative containing 5% acetic acid overnight at room temperature.
  8. Rinse in running water ————————— 2 minutes
  9. Place in Gram’s Iodine ————————— 15 minutes

10. Rinse in running water ————————— 1 minute

11. Place in 5% sodium thiosulfate ——————– 3 minutes

12. Wash in running water ————————— 10 minutes

13. Place in 0.25% potassium permanganate ——— 5 minutes

14. Rinse in running water ————————— 1 minute

15. Place in 5% oxalic acid ————————— 1 minute

16. Stain in PTAH at room temp ——————— Overnight

17. Dehydrate in 95% alcohol————————- 30 seconds

18. Dehydrate in 100% alcohol———————– 30 seconds

19. Dehydrate in 100% alcohol———————- 30 seconds

20. Dehydrate in 100% alcohol———————- 30 seconds

21. Clear in xylene (3)——————————- 2 minutes (re-use)

22. Clear in xylene (4)——————————- 5 minutes (re-use)

Results:

Cross-striations, fibrin – Blue

Nuclei – Blue

Collagen – Red-Brown