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Antibody Discoveries Customized for You

If you need immunohistochemistry for your project, we have years of experience working with both known and unknown antibody protocols.  We can get your project done fast with great results at a fraction of the price.  Call or email us today!

Picture of Mouse Stomach - Antibody HMGB1
Mouse Stomach – Antibody HMGB1
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10 Best Processing Protocols for Rat and Mouse

These days all seem to run together when all you work on is Rat and mouse tissues.  The odd thing is you get use to it pretty quickly.  There are so many ways to process tissues that after dealing with them in great quantity, it seems like almost total recall is inevitable.  Sometimes the processes for one set of tissues for a particular study just do not work for another.  In this case one must try new-made up protocols until you find something that works.  Usually the tissues of Rat and mouse are so similar that they can be substituted for one another.  For certain tissues such as bone and eyes, that may or may not be the case.  Sometimes there are cases where the client prefers a particular protocol and can provide that for you.  I have seen protocols that I believe were passed down from generation to generation, so to speak, that were used originally for hand processing or an autotechnicon.  The process was tried and true for those set circumstances once and never modified or tested again afterwords.  The client may vehemently insist on using their protocol even when confronted with evidence that another protocol works better.  In these times I just try to put myself in their shoes and see from their point of view.  In most cases their protocol needs only minor modifications to an already set up protocol in use.  There was one time that a researcher would not change until the new process was proven with control tissues processed, cut and stained for review.  The old process was 23 hours long for tissues 1cm x 1cm. So here are the processing protocols that we use for all of our mouse and rat tissue, they may help you through your project.  Just as a heads up, the machine we use is a new TBS ATP-120.  This is a new machine with all the bells and whistles.  Prior to this we ran the same protocols on a shandon hypercenter XP with almost exact results.  All the protocols are in PDF format and may be downloaded and printed.

Process 1 (Rat & Mouse)

Process 2 (Rat & Mouse)

Eyes (Mouse)

Eyes (Rat & Mouse)

Fetal toes (Rat & Mouse)

Toes (Rat & Mouse)

Toes 2 (Rat, Mouse & Other)

Skin (Rat&Mouse)

Varying age

Bouins (Rat & Mouse)

 

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Woodland Pharmaceuticals Metastasis models

Hello everyone, we are proud to announce that we have been working with Woodland Pharmaceuticals in their project to identify a liver to lung metastasis model (MHCC97H) and are evaluating the lung cancer SQ A549 for lung lesions as well. At this point Woodland Pharmaceuticals is establishing a stable luciferase to express tumor cell lines with their partner SBH Sciences so metastasis can be quantified. They have positive control compounds that will mechanistically block most/all of this metastasis.  We have done all their histology so far and have much more to do.  Follow us with this exciting project.

Here are some of the pictures.

Primary tumor 10x
Primary tumor 10x
Mouse lung metastasis 40x
Mouse lung metastasis 40x
Liver metastasis 20x
Liver metastasis 20x
Liver metastasis 40x
Liver metastasis 40x

 

 

 

 

 

 

 

 

If you have LinkedIn account, you can take look at this exciting project at;  http://www.linkedin.com/company/2696725?trk=tyah

 

 

An Expert Histology Service

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Histology service

histology service lipid

We have an extensive array of histology knowledge, from years of working in clinical, research and academia histology labs.  Our techniques have been modified to give you excellent results and turnaround times.  The desire to help your causes, through research advance faster through the end stage of a histology service drives our success. We understand that your research is the most important thing in the world and our job is to make your life easy by doing everything right on our end within a reasonable time.

Do to this, we have built our lab for efficiency and quality.  All specimens come in and leave in a circular fashion. This eliminates redundancy and waste by creating checks and balances that make a lean lab.  We believe, it’s our job to make you look good.

Come see what we can do for you.