Posted on 3 Comments

Masson’s Trichrome Green Protocol

Masson’s Trichrome Green Protocol

1. De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
2. De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
3. Clear slides in 100% alcohol———————– 2 minutes
4. Clear slides in 100% alcohol———————– 2 minutes
5. Hydrate slides in 95% alcohol———————- 2 minutes
6. Hydrate slides in running water——————– 5 minutes
7. Bouin’s Fixative (60 Celsius)———————- 30 minutes (re-use)
8. Wash in running water—————————— 5 minutes
9. Weigerts——————————————- 10 minutes (dump)
10. Wash in running water—————————— 5 minutes
11. Biebrich scarlet acid fuchsin———————- 5 minutes (re-use)
12. Rinse quickly————————————– 30-60 seconds
13. Phostung-Phosmoly acid (PTA-PMA)——————- 5 minutes (re-use)
14. No rinse
15. 2.5% Fast green———————————— 5 minutes (re-use)
16. Rinse quickly ————————————- 30 seconds
17. 1% Glacial acetic acid—————————– 2 minutes (re-use)
18. Dehydrate in 95% alcohol————————— 30 seconds
19. Dehydrate in 100% alcohol————————– 1 minute
20. Dehydrate in 100% alcohol————————– 2 minutes
21. Dehydrate in 100% alcohol————————– 2 minutes
22. Clear in xylene (3)——————————– 2 minutes (re-use)
23. Clear in xylene (4)——————————– 5 minutes (re-use)

Results:
Nuclei——————— Black to purple
Cytoplasm- —————- Shades of pink, red
Erythrocytes and myelin—- Scarlet
Collagen fibers———— Strong bluish-green
Mucus———————- Lighter green

Posted on Leave a comment

Masson’s Trichrome Blue Protocol

Masson’ Trichrome Blue
Safety equipment: Work under a hood with lab coat, gloves, and glasses.
1. De-paraffin slides in xylene (1)———————- 2 minutes (re-use)
2. De-paraffin slides in xylene (2)———————- 2 minutes (re-use)
3. Clear slides in 100% alcohol————————– 2 minutes
4. Clear slides in 100% alcohol————————– 2 minutes
5. Hydrate slides in 95% alcohol————————- 2 minutes
6. Hydrate slides in running water———————– 5 minutes
7. Bouins at 60 degrees Celsius————————– 1 hour (re-use)
8. Wash in running water ——————————– 10 minutes
9. Weigert’s Hematoxylin (make fresh) —————— 10 minutes (dump)
10. Wash in running water ——————————– 10 minutes
11. Biebrich Scarlet Acid Fuchsin————————- 10 minutes (re-use)
12. Rinse in running water——————————– 2 minutes
13. Phosphomolybdic / phosphotungstic acid—————- 10 minutes (re-use)
14. No rinse, goes directly into blue
15. Analine Blue—————————————— 10 minutes (re-use)
16. Rinse in running water——————————– 1-2 minutes
17. 1% Glacial Acetic Acid——————————– 5 minutes (re-use)
18. Dehydrate in 95% alcohol—————————— 1 minute
19. Dehydrate in 95% alcohol—————————— 1 minute
20. Dehydrate in 100% alcohol—————————– 1 minute
21. Dehydrate in 100% alcohol—————————– 2 minutes
22. Dehydrate in 100% alcohol—————————– 2 minutes
23. Clear in xylene (3)———————————– 2 minutes (re-use)
24. Clear in xylene (4)———————————– 5 minutes (re-use)

Results:
Nuclei- Black
Cytoplasm, keratin, muscle fiber and intercellular fibers- Red
Collagen- Blue

Posted on

Histology Lab

  Histologistics is a full service histology lab. We provide excellent quality histology to clinical facilities, veterinarians and researchers . We can help you with anything.  Please contact us with your histology needs.