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Beta-Catenin Staining

Mouse testicle CTNNB1

We offer many antibody staining services, like working up protocols for unknown antibodies. This was the case for the Beta-Catenin (CTNNB1) antibody from Biolegend.

Our Beta-catenin antibody works in human, mouse and rat tissues.  Both normal and abnormal tissues exhibit positive staining.

 

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Cytomegalovirus Works on First Run

This past week, we worked up two new antibodies. One the Novusbio CMV antibody, that will be discussed here and IGF1 which will be detailed in another post.  For the CMV antibody test, we used positive human infected tissue control slides (paraffin) made by us.  This is for a larger client project that will hopefully continue for many years.

Cytomegalovirus
Human Placenta CMV 10x

We’ve had a great deal of good success with antibodies lately, getting them to work on the first run and this one was no exception.  We used the company recommended dilution along with 2 others, just to make sure theirs was the best.  Then we used our signature antibody work-up procedure, a range of different HIER, blocking, and reagent chemicals along with various times and temperatures.  This procedure has been used to decipher many of the antibodies without protocols.

CMV IHC
Human Placenta CMV
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Colon Adenocarcinoma

Colon Cancer

Pathology of colon cancer as seen through the eyes of a histologist.  How many different ways are there to visualize cancer?  The more the better!

Hematoxylin & Eosin (our recipe)

HMGB1 Antibody – According to the The Human Protein Atlas HMGB1 protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration.

CD8 Antibody in the Human Protein Atlas ( CD8A) describes it as a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.

Indoleamine Antibody – This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity (IDO1).

Perforin Antibody – This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis (Perforin).

Colon cancer
Colon Adenocarcinoma Perforin Antibody

 

Note: All tissues were bought, cut and stained at Histologistics Inc as positive control slides.  These are not part of any study.

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Antibody Discoveries Customized for You

If you need immunohistochemistry for your project, we have years of experience working with both known and unknown antibody protocols.  We can get your project done fast with great results at a fraction of the price.  Call or email us today!

Picture of Mouse Stomach - Antibody HMGB1
Mouse Stomach – Antibody HMGB1