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Calcium Staining in Histology

There are many stains out there to histologically stain for calcium but we use primarily 2.  They both give different color results and use different chemicals.  The first is called Alizarin Red S.  This stain can be visualized with or without a polarizing microscope.  The polarized microscope makes the calcium deposits much brighter (see picture below).  As the picture suggests, we also carry positive control slides for this and other calcium stains.  We like this one the best because it is a much quicker stain that can be completed in 25 minutes using paraffin slides.  The chemicals necessary for the stain are alizarin Red S powder and ammonium hydroxide.  You also need a pH meter to get the solution to a pH range of 4.1-4.3.

The other stain is Von Kossa‘s method for calcium.  This is a much longer stain (1 hour 41 minutes) that uses silver nitrate to turn the calcium salts black.  To achieve good results with this stain, the tissue sections must be exposed to direct sunlight, a UV lamp or a 100 watt lamp light while immersed in the silver nitrate.  In our opinion this stain is more finicky and easier to foul up than the Alizarin Red stain.  Unlike most silver protocols, the glassware in this protocol will not turn black.  The Von Kossa solution protocol will be posted on the protocol solutions the day after today.

Human Kidney  Von Kossa
Human Kidney Von Kossa



How to clean glassware after doing silver stains:

After the stain is complete and the controls tissues have been verified, the toning solution (gold chloride) can be used to clean all glassware.  Keep in mind that this solution is very toxic and should be disposed of properly (not down the sink).


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Positive Control Slides (Amazing Prices)

Take a look at these high quality control slides.  We have human and animal controls with prices that cannot be beat.  All controls are considered custom orders and will be cut after orders have been placed.  We have an abundant supply of animal tissue controls and a limited supply of human controls.  They are all at the lowest prices guaranteed.


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Melanin Slide Controls

Are you looking for Melanin control tissues?  We have melanin control slides that are located in human skin and can be seen in the H&E stain.  These are high quality slides that can be cut to your specification with negative on the same slide or not.  These can be used for special stains or IHC.  Do not delay, these are selling at the lowest prices available and will sell quickly.  Call or email us today.

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Helicobacter Pylori Control Tissue Sale

We have an abundance of human helicobacter pylori (HP) tissue.  This is great for immunohistochemistry control tissue.  While trying out a potential tissue supplier, we order way to much tissue and now have no room for it all.  To rectify this we are practically giving it away.  All slides for $2.00 each, minimum 50 slides please.  All orders are cut to order and includes the first and last slides stained with H&E.  Slide boxes are extra but you can save money by sending us your own slide box.  Please help us reduce our inventory!

Sale ends when the tissue is gone.

Human colon IHC Helicobacter Pylori
Human colon IHC Helicobacter Pylori

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