Tag: histology special stains

AFB, alcian blue, alizarin Red S, Bielschowsky, congo red, Copper Rhodanine, giemsa, Gram, GMS, LFB, Masson’s trichrome, movat’s, oro, Orcein, PAS, picro sirius red, safranin O, t-blue, vvg.

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Free Control Slides

Histology Control Slides

Beginning this week, all 22ct histology control slides (see link below) bought will now come with 3 extra unstained slides FREE. Buy 22 get 25 controls.
Histology Control Slides

We have 24 different human histology control types to choose. Mouse and rat normal tissues are also available as control slide sets (inquire). Some controls like Fite, fungus, gram and helicobacter pylori are four of our most popular controls. They go fast, get them before they’re gone!

This promotion (3 free slides) will be species specific but not necessarily same tissue type. We will try to match same controls as bought but if they are not available, we will throw in another validated control type. If you order hospital grade human tissues you will get 3 similar unstained slides, free. Likewise, if you order Mouse or Rat 22ct, you will get 3 free mouse or rat slides respectively.

Extra slides will be marked with either our slide printer or hand written. Extra slides may or may not be sequentially ordered and will be placed at the back of each box.

Free slides are not guaranteed and will discontinue at anytime. 100ct boxes not included in the promotion.

Contact us at 508.461.7207

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Elastic Fiber Pictures

Human skin VVG 20x
Human skin VVG 20x

Human skin VVG 10x
Human skin VVG 10x

Here are the other 2 pictures that were going to be in the previous post, plus 6 more.  The other 6 are also 10x,20x, and 40x of 2 different tissues.  I can not imagine what life would be like without flexibility.

Aorta Verhoeff Van Gieson 10x
Aorta Verhoeff Van Gieson 10x

Aorta Verhoeff Van Gieson 20x
Aorta Verhoeff Van Gieson 20x

Aorta Verhoeff Van Gieson 40x
Aorta Verhoeff Van Gieson 40x

Human Uterus Verhoeff Van Gieson 10x
Human Uterus Verhoeff Van Gieson 10x

Human Uterus Verhoeff Van Gieson 20x
Human Uterus Verhoeff Van Gieson 20x

Human Uterus Verhoeff Van Gieson 40x
Human Uterus Verhoeff Van Gieson 40x

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Verhoeff Van Gieson Stain

Safety equipment: Work under a hood with lab coat, gloves, and glasses.

  1. De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
  2. De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
  3. Clear slides in 100% alcohol———————- 2 minutes
  4. Clear slides in 100% alcohol———————- 2 minutes
  5. Hydrate slides in 95% alcohol——————— 2 minutes
  6. Hydrate slides in running water——————- 5 minutes
  7.  VVG working solution—————————— 60 minutes (re-use)
  8. Wash in running water—————————— 2 minutes
  9. Differentiate sections microscopically in 2% ferric chloride until the elastic fibers are distinct and the background is colorless to light grey.  If the sections are differentiated too far, re-stain. (see notes below)
  10. Rinse in DH2O—————————————- 1 minute
  11. Sodium thiosulfate———————————– 1 minute (re-use)
  12. Wash in running water—————————— 5 minutes
  13. Counterstain in Van Gieson’ s——————— 1 minute (re-use)
  14. Dehydrate in 95% alcohol————————– 1 minute
  15. Dehydrate in 95% alcohol———————— 1 minute
  16. Dehydrate in 100% alcohol———————- 1 minute
  17. Dehydrate in 100% alcohol———————- 2 minutes
  18. Dehydrate in 100% alcohol———————- 2 minutes
  19. Clear in xylene (3)———————————- 2 minutes
  20. Clear in xylene (4)———————————- 5 minutes
  21. Coverslip 

Results:

Elastic Fibers —————————————- Black

Background —————————————— Yellow

Notes:

  1. It is easy to over differentiate.  If the background is completely colorless so that a clear yellow counterstain is obtained, the section may be over differentiated.  It is probably better to err on the side of under differentiating.
  2. Over differentiated sections can be re-stained.
  3. Do not prolong the Van Gieson stain; picric acid could differentiate the stain further.
  4. Be sure to follow the order of adding all reagents when making the Verhoeff’s solution.
  5. To clean the Verhoeff’s stain out of the jar, wash with 2% ferric chloride solution for a few minutes.
  6. For optimal results differentiate the control slide first.

Porcine Aorta VVG 40x

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Special Stain Solution Protocols Page

Hello,  We have starting adding our solution protocols on a new page.  These are all the protocols that we use now. A professional histology service.

http://histologistics.com/histology/special-stain-solution-protocols/

An Expert Histology Service