Here are the other 2 pictures that were going to be in the previous post, plus 6 more. The other 6 are also 10x,20x, and 40x of 2 different tissues. I can not imagine what life would be like without flexibility.
Elastic Fiber Pictures
Tag: histology special stains
AFB, alcian blue, alizarin Red S, Bielschowsky, congo red, Copper Rhodanine, giemsa, Gram, GMS, LFB, Masson’s trichrome, movat’s, oro, Orcein, PAS, picro sirius red, safranin O, t-blue, vvg.
Verhoeff Van Gieson Stain
Safety equipment: Work under a hood with lab coat, gloves, and glasses.
- De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
- De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
- Clear slides in 100% alcohol———————- 2 minutes
- Clear slides in 100% alcohol———————- 2 minutes
- Hydrate slides in 95% alcohol——————— 2 minutes
- Hydrate slides in running water——————- 5 minutes
- VVG working solution—————————— 60 minutes (re-use)
- Wash in running water—————————— 2 minutes
- Differentiate sections microscopically in 2% ferric chloride until the elastic fibers are distinct and the background is colorless to light grey. If the sections are differentiated too far, re-stain. (see notes below)
- Rinse in DH2O—————————————- 1 minute
- Sodium thiosulfate———————————– 1 minute (re-use)
- Wash in running water—————————— 5 minutes
- Counterstain in Van Gieson’ s——————— 1 minute (re-use)
- Dehydrate in 95% alcohol————————– 1 minute
- Dehydrate in 95% alcohol———————— 1 minute
- Dehydrate in 100% alcohol———————- 1 minute
- Dehydrate in 100% alcohol———————- 2 minutes
- Dehydrate in 100% alcohol———————- 2 minutes
- Clear in xylene (3)———————————- 2 minutes
- Clear in xylene (4)———————————- 5 minutes
- Coverslip
Results:
Elastic Fibers —————————————- Black
Background —————————————— Yellow
Notes:
- It is easy to over differentiate. If the background is completely colorless so that a clear yellow counterstain is obtained, the section may be over differentiated. It is probably better to err on the side of under differentiating.
- Over differentiated sections can be re-stained.
- Do not prolong the Van Gieson stain; picric acid could differentiate the stain further.
- Be sure to follow the order of adding all reagents when making the Verhoeff’s solution.
- To clean the Verhoeff’s stain out of the jar, wash with 2% ferric chloride solution for a few minutes.
- For optimal results differentiate the control slide first.
Special Stain Solution Protocols Page
Hello, We have starting adding our solution protocols on a new page. These are all the protocols that we use now. A professional histology service.
http://histologistics.com/histology/special-stain-solution-protocols/
An Expert Histology Service
How To Detect Alzheimer’s Disease Using Histology
Dave Adams and Ben Snyder created the first transgenic mouse model in 1995. Histology played a role in the detection of the amyloid plaques surrounded by dystrophic neurites, and show profound synaptic loss, neurofibrillary tangle formation and gliosis. The beta amyloid and Tau proteins are detected by antibodies used in immunohistochemistry. There are a whole host of different special stains for neuroglia, astrocytes, myelin sheath, neurofibril tangles and senile plaques. The Bielschowsy stain shows neurofibrils and senile plaques black while the background is yellow-brown.
http://www.nature.com/nature/journal/v373/n6514/abs/373523a0.html
An Expert Histology Service