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Elastic Fiber Pictures

Human skin VVG 20x
Human skin VVG 20x
Human skin VVG 10x
Human skin VVG 10x

Here are the other 2 pictures that were going to be in the previous post, plus 6 more.  The other 6 are also 10x,20x, and 40x of 2 different tissues.  I can not imagine what life would be like without flexibility.

Aorta Verhoeff Van Gieson 10x
Aorta Verhoeff Van Gieson 10x
Aorta Verhoeff Van Gieson 20x
Aorta Verhoeff Van Gieson 20x
Aorta Verhoeff Van Gieson 40x
Aorta Verhoeff Van Gieson 40x
Human Uterus Verhoeff Van Gieson 10x
Human Uterus Verhoeff Van Gieson 10x
Human Uterus Verhoeff Van Gieson 20x
Human Uterus Verhoeff Van Gieson 20x
Human Uterus Verhoeff Van Gieson 40x
Human Uterus Verhoeff Van Gieson 40x
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Verhoeff Van Gieson Stain

Safety equipment: Work under a hood with lab coat, gloves, and glasses.

  1. De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
  2. De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
  3. Clear slides in 100% alcohol———————- 2 minutes
  4. Clear slides in 100% alcohol———————- 2 minutes
  5. Hydrate slides in 95% alcohol——————— 2 minutes
  6. Hydrate slides in running water——————- 5 minutes
  7.  VVG working solution—————————— 60 minutes (re-use)
  8. Wash in running water—————————— 2 minutes
  9. Differentiate sections microscopically in 2% ferric chloride until the elastic fibers are distinct and the background is colorless to light grey.  If the sections are differentiated too far, re-stain. (see notes below)
  10. Rinse in DH2O—————————————- 1 minute
  11. Sodium thiosulfate———————————– 1 minute (re-use)
  12. Wash in running water—————————— 5 minutes
  13. Counterstain in Van Gieson’ s——————— 1 minute (re-use)
  14. Dehydrate in 95% alcohol————————– 1 minute
  15. Dehydrate in 95% alcohol———————— 1 minute
  16. Dehydrate in 100% alcohol———————- 1 minute
  17. Dehydrate in 100% alcohol———————- 2 minutes
  18. Dehydrate in 100% alcohol———————- 2 minutes
  19. Clear in xylene (3)———————————- 2 minutes
  20. Clear in xylene (4)———————————- 5 minutes
  21. Coverslip 

Results:

Elastic Fibers —————————————- Black

Background —————————————— Yellow

Notes:

  1. It is easy to over differentiate.  If the background is completely colorless so that a clear yellow counterstain is obtained, the section may be over differentiated.  It is probably better to err on the side of under differentiating.
  2. Over differentiated sections can be re-stained.
  3. Do not prolong the Van Gieson stain; picric acid could differentiate the stain further.
  4. Be sure to follow the order of adding all reagents when making the Verhoeff’s solution.
  5. To clean the Verhoeff’s stain out of the jar, wash with 2% ferric chloride solution for a few minutes.
  6. For optimal results differentiate the control slide first.

Porcine Aorta VVG 40x

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Special Stain Solution Protocols Page

Hello,  We have starting adding our solution protocols on a new page.  These are all the protocols that we use now. A professional histology service.

http://histologistics.com/histology/special-stain-solution-protocols/

An Expert Histology Service

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How To Detect Alzheimer’s Disease Using Histology

 Dave Adams and Ben Snyder created the first transgenic mouse model in 1995. Histology played a role in the detection of the amyloid plaques surrounded by dystrophic neurites, and show profound synaptic loss, neurofibrillary tangle formation and gliosis.  The beta amyloid and Tau proteins are detected by antibodies used in immunohistochemistry. There are a whole host of different special stains for neuroglia, astrocytes, myelin sheath, neurofibril tangles and senile plaques.  The Bielschowsy stain shows neurofibrils and senile plaques black while the background is yellow-brown.

http://www.nature.com/nature/journal/v373/n6514/abs/373523a0.html

Beilschowsky Special Stain on Mouse Brain For Neurofibrils and Senile Plaques 20x
Beilschowsky Special Stain on Mouse Brain For Neurofibrils and Senile Plaques 20x

 

 

 

 

 

An Expert Histology Service