Posted on 2 Comments

What does this stain for?

We have been testing a new fluorescent compound for months.  At first we thought it might stain GAGs because the commonalities of the tissue stained pointed to this.  Today we stained the same tissues but used a different fluorescent scope to view the results.  They were varied and inconsistent from the original.  My best guess to what tissues are being highlighted are red blood cells.  Take a look at the pictures.  Will anyone help us figure out these structures?

 

Human Cord fluorescent blue 10x
Human Cord fluorescent blue 10x
Human Cord fluorescent blue 20x
Human Cord fluorescent blue 20x
Human Heart fluorescent 20x
Human Heart fluorescent 20x
Human Heart fluorescent 40x
Human Heart fluorescent 40x
Human Heart fluorescent 40x
Human Heart fluorescent 40x
Human Heart fluorescent 20x
Human Heart fluorescent 20x
Human Placenta fluorescent blue 10x
Human Placenta fluorescent blue 10x
Human skin fluorescent 10x
Human skin fluorescent 10x
Human skin fluorescent 40x
Human skin fluorescent 40x
Rat colon fluorescent blue 20x
Rat colon fluorescent blue 20x

 

Posted on 4 Comments

Special stains protocols

I am trying to figure out what to do about the special stains protocols.  There are about 35 of them.  Putting them on the front page does not seem to be efficient.   I would like them all to be searchable and downloadable, maybe on a different page.  What do you think, does it make a difference?

Posted on 3 Comments

Alcian Blue / PAS Stain Protocol

Here is the protocol for alcian blue periodic acid schiff  (AB /PAS).  At the bottom of the post is the link for printing.

Control tissue: Large Intestine

Rat colon PASAB2

  1. De-paraffin slides in xylene (1) ———— 2 minutes (re-use)
  2. De-paraffin slides in xylene (2)———— 2 minutes (re-use)
  3. Clear slides in 100% alcohol————— 2 minutes
  4. Clear slides in 100% alcohol————— 2 minutes
  5. Hydrate slides in 95% alcohol————- 2 minutes
  6. Hydrate slides in running water———-  5 minutes
  7. Place slides in 3% Acetic Acid————- 3 minutes (re-use)
  8. Alcian Blue ———————————– 30 minutes (re-use)
  9. Wash in running water ——————— 10 minutes
  10. Oxidize in 0.5% periodic acid ————- 10 minutes (re-use)
  11. Wash in running water ——————— 5 minutes
  12. Schiff reagent (room temp)—————- 20 minutes (re-use)
  13. Wash in running water ——————— 10 minutes
  14. Dehydrate in 95% alcohol—————— 30 seconds
  15. Dehydrate in 100% alcohol—————- 1 minute
  16. Dehydrate in 100% alcohol—————- 2 minutes
  17. Dehydrate in 100% alcohol—————- 2 minutes
  18. Clear in xylene (3)————————— 2 minutes (re-use)
  19. Clear in xylene (4)————————— 5 minutes (re-use)

Results: Polysaccharides and Mucosubstances containing hexoses or deoxyhexoses with vicinal glycol groups – Magenta to Red

Hyaluronic acid, sialomucins and all but the most strongly acidic sulfated mucosubstances – Blue

PAS Alcian Blue

Posted on Leave a comment

Histology service

histology service lipid

We have an extensive array of histology knowledge, from years of working in clinical, research and academia histology labs.  Our techniques have been modified to give you excellent results and turnaround times.  The desire to help your causes, through research advance faster through the end stage of a histology service drives our success. We understand that your research is the most important thing in the world and our job is to make your life easy by doing everything right on our end within a reasonable time.

Do to this, we have built our lab for efficiency and quality.  All specimens come in and leave in a circular fashion. This eliminates redundancy and waste by creating checks and balances that make a lean lab.  We believe, it’s our job to make you look good.

Come see what we can do for you.