Posted on Leave a comment

Frozen Tissue Analysis (cryostat sectioning)

Leica CM 3050 Cryostat (outside)
Leica CM 3050 Cryostat (outside)
Leica CM 3050 Cryostat (inside)
Leica CM 3050 Cryostat (inside)

Ever heard of Mohs surgery, also known as chemosurgery?  The cryostat is used in conjunction with surgery where the surgeon removes skin cancer and has it processed in real time by the technician sitting at the cryostat.  All tissue pieces are frozen, cut, stained and then read by a pathologist while the surgeon and patient wait for the results.  The tissue is cut and analyzed in as few as 10-15 minutes.   If the outside edges of the cut tissues contain cancer cells, then the surgeon must take deeper sections and the process is repeated.  If there are no cancer cells then the work is done.

Is cutting on the cryostat a major pain in your hands?  I know it can be.  The cryogenic part of histology is not something that everyone enjoys.  The fact that the tissue is not fixed or is still live and therefore contagious, can be a major concern for anyone having to cut the tissue.  It is not always the case, some tissues are fixed and then infused with 30% sucrose for easier sectioning.  This is not usually the biggest concern surrounding cryostat work.  When I think about the requirements necessary to cut tissue under very cold temperatures, it makes the hair on my arm raise in tense anticipation.  Truthfully I’m sure why I get this sensation.  I enjoy cutting most tissue on the cryostat and view it as a challenge to create perfect sections.  Maybe it’s more the anatomical positions that one must contort, in order to cut on this type of machine.  Or maybe it’s the extreme temperatures of -10 to -50 celsius, that get under my skin.   For those of you who may not know what this entails and or feels like, here is a short video.  If you have trouble cutting and need to know what temperature your tissue needs to be cut at, the link below may help.  We consider ourselves experts in this area because we have cut so many kinds of tissues so many different ways, so if you need excellent cryostat services we are here for you. 

http://www.youtube.com/watch?v=d43LFVV3h6w

http://histologistics.com/wp-content/uploads/2013/07/cryostat-tissue-cutting-temp-guide.pdf

 

Posted on Leave a comment

Small Business Histology

Come experience our extremely skilled and experienced technical team to show what your experiment results could look like.    Try us today.

background.jpg

 

 

 

 

 

 

An Expert Histology Service

Posted on Leave a comment

Histology service

histology service lipid

We have an extensive array of histology knowledge, from years of working in clinical, research and academia histology labs.  Our techniques have been modified to give you excellent results and turnaround times.  The desire to help your causes, through research advance faster through the end stage of a histology service drives our success. We understand that your research is the most important thing in the world and our job is to make your life easy by doing everything right on our end within a reasonable time.

Do to this, we have built our lab for efficiency and quality.  All specimens come in and leave in a circular fashion. This eliminates redundancy and waste by creating checks and balances that make a lean lab.  We believe, it’s our job to make you look good.

Come see what we can do for you.

Posted on

Histology Lab

  Histologistics is a full service histology lab. We provide excellent quality histology to clinical facilities, veterinarians and researchers . We can help you with anything.  Please contact us with your histology needs.