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There are 5 electrolyte molar concentrations to stain for. This week I stained for 3 of them. The alcian blue stain works well to detect it.
Every concentration had different results.
0.06 – Carboxylated and sulfated mucosubstances and mucins.
0.3 – Weakly and strongly sulfated mucosubstances and mucins.
0.5 – Strongly sulfated mucosubstances and mucins.
0.7 – Highly sulfated connective tissue mucosubstances.
0.9 Keratan sulfate.