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Histology service

histology service lipid

We have an extensive array of histology knowledge, from years of working in clinical, research and academia histology labs.  Our techniques have been modified to give you excellent results and turnaround times.  The desire to help your causes, through research advance faster through the end stage of a histology service drives our success. We understand that your research is the most important thing in the world and our job is to make your life easy by doing everything right on our end within a reasonable time.

Do to this, we have built our lab for efficiency and quality.  All specimens come in and leave in a circular fashion. This eliminates redundancy and waste by creating checks and balances that make a lean lab.  We believe, it’s our job to make you look good.

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Glycosaminoglycans (GAGs)

What are the components of connective tissue?

They are ground substance, extracellular fibers and cellular elements. Ground substance is a matrix that allows diffusion and binds fibers and other tissue elements together.

Components of ground substance are glycoaminoglycans (GAGS), water, calcium.

4 functions of the ground substance are glue, lubrication, barrier, tensil strength.

What are the 2 groups of ground substance,  glycoaminoglycans (GAGS) and glycoproteins.

What are GAGS, they are structural proteins bound to protein core to create a bottle brush structure.

2 types of GAGS are hyaluronic acid and he sulfates.

They have a highly negative because of the sulfate group.

Explain how hyaluronic acid differs from the rest of the GAGS.

Longer chain and forms the base of the bottle brush that the core protein binds to.

Describe a proteoglycan monomer.

A bunch of GAGS around a central protein linked to a hyaluronic acid.

Components of ground substance are: sulfated glycoaminoglycans; heparin sulfate, dermatan sulfate, keratin sulfate, chondroitin sulfate (GAGS sulfated).

What are the function of gags.

They electrostatically bind to collagen for strength, trap water to make a gel that enables tissues to withstand compression.

They are a matrix for cells and blood vessels and a diffusion medium.

There are 5 electrolyte  molar concentrations to stain for. This week I stained for 3 of them. The alcian blue stain works well to detect it.

Every concentration had different results.
0.06 – Carboxylated and sulfated mucosubstances and mucins.
0.3 – Weakly and strongly sulfated mucosubstances and mucins.
0.5 – Strongly sulfated mucosubstances and mucins.
0.7 – Highly sulfated connective tissue mucosubstances.
0.9 Keratan sulfate.

Here are the results:

Rat colon 0.5 10x
Rat colon 0.5 10x
Rat Colon AB 0.7 10x
Rat Colon AB 0.7 10x
Rat colon AB 0.9 10x
Rat colon AB 0.9 10x
Rat knee joint AB 0.5
Rat knee joint AB 0.5