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Free Control Slides

Histology Control Slides

Beginning this week, all 22ct histology control slides (see link below) bought will now come with 3 extra unstained slides FREE. Buy 22 get 25 controls.
Histology Control Slides

We have 24 different human histology control types to choose. Mouse and rat normal tissues are also available as control slide sets (inquire). Some controls like Fite, fungus, gram and helicobacter pylori are four of our most popular controls. They go fast, get them before they’re gone!

This promotion (3 free slides) will be species specific but not necessarily same tissue type. We will try to match same controls as bought but if they are not available, we will throw in another validated control type. If you order hospital grade human tissues you will get 3 similar unstained slides, free. Likewise, if you order Mouse or Rat 22ct, you will get 3 free mouse or rat slides respectively.

Extra slides will be marked with either our slide printer or hand written. Extra slides may or may not be sequentially ordered and will be placed at the back of each box.

Free slides are not guaranteed and will discontinue at anytime. 100ct boxes not included in the promotion.

Contact us at 508.461.7207

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Special Stain Solution Protocols Page

Hello,  We have starting adding our solution protocols on a new page.  These are all the protocols that we use now. A professional histology service.

http://histologistics.com/histology/special-stain-solution-protocols/

An Expert Histology Service

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Special stains protocols

I am trying to figure out what to do about the special stains protocols.  There are about 35 of them.  Putting them on the front page does not seem to be efficient.   I would like them all to be searchable and downloadable, maybe on a different page.  What do you think, does it make a difference?

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Acid Fast Bacilli (AFB) Protocol

Here is the protocol for acid fast bacilli (AFB).  At the bottom of the post is the link for printing.

Control Tissue : Tissue with AFB present

Human Kidney AFB
Human Kidney AFB

  1.  De-paraffin slides in xylene (1) ——————- 2 minutes (re-use)
  2.   De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
  3.   Clear slides in 100% alcohol———————- 2 minutes
  4.   Clear slides in 100% alcohol———————- 2 minutes
  5.   Hydrate slides in 95% alcohol——————– 2 minutes
  6.   Hydrate slides in running water—————— 5 minutes
  7.   Carbol Fuchsin————————————– 45 minutes (re-use)
  8.   Wash in running water—————————– 1 minute
  9.   Decolorize in 1% acid alcohol——————— until sections are pale pink
  10.   Wash in running water—————————– 8 minutes
  11.   Counterstain with working methylene blue—–1 dip (re-use (good for 5 days))
  12.   Rinse quickly—————————————- 30 seconds
  13.   Dehydrate in 95% alcohol———————— 1 minute
  14.   Dehydrate in 100% alcohol———————- 1 minute
  15.   Dehydrate in 100% alcohol———————- 2 minutes
  16.   Clear in xylene (3)——————————— 2 minutes (re-use)
  17.   Clear in xylene (4)——————————— 5 minutes (re-use)

Results:

Acid-fast bacilli —————- Bright Red

Erythrocytes——————– Yellowish Orange

Other tissue elements——- Pale blue

Acid Fast Bacilli