Description

This protocol is intended for use with formalin fixed paraffin embedded (FFPE) tissues.

This is Abcam’s antibody and is a mouse monoclonal and targets mouse, rat, rabbit, and human species.  Human and mouse tissues are all that I tested for this protocol.

 

Be sure to also run species specific, known positive control slides, like normal human tonsil.

This protocol gives:

• Specific antibody with clone and manufacturer
• The antibody concentration (dilution)
• The species tested
• Types of tissues for positive controls
• Antigen retrieval method with pH
• The secondary antibody species
• Primary and secondary antibody temperatures and times
• DAB color used
• Counterstain used
• All accessory reagents used
• Antibody staining pattern with pictures