CD3 Antibody Protocol


CD3 Antibody Protocol


This Novus Biological antibody was tested on Human and canine tissues but according to the data sheet, also works on equine, feline and primate tissues.  The human tonsil and canine lymph node tissues look great for this antibody.

Testing of equine, feline and primate tissues was not feasible.

This protocol gives:

  • Specific antibody with clone and manufacturer
  • The antibody concentration (dilution)
  • The species tested
  • Types of tissues for positive controls
  • Antigen retrieval method with pH
  • The secondary antibody species
  • Primary and secondary antibody temperatures and times
  • DAB color used
  • Counterstain used
  • All accessory reagents used
  • Antibody staining pattern with pictures



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