Trichrome Positive Control slides

These are high quality histopathology positive control tissues, positive for Trichrome staining.  Default tissue is skin. 

The first and last slide (2) are stained (Gomori’s One step Trichrome) for authenticity before sending to you. These tissues are designed and specified for special stains, they are not optimal for IHC.

Human tissue. Cut at 5um, placed at top third of slide.

All tissues types are placed on positively charged adhesive slides.  We have rigorously tested these slides for routine, special, staining without issues.  All slides have the stain type, slide, block and lot number printed using the signature slide mate.  Unstained slides are air dried (not baked) unless requested, for a minimum of 48 hours before shipment. Every stained slide is microscopically checked for positive material.  

Current turnaround times for control slides – Cut to order – 1 – 4 weeks

All slide sets are sent with a 30-day guarantee and will be replaced on request.

 

Product Codes

CS-TRIS25 – 22ct box, 2 stained, 20 unstained – $107.80 – Skin

CS-TRIS100 – 100ct box, 2 stained, 98 unstained – $280.00 – Skin

Shipping

All shipping is done through FedEx and or UPS and is charged to the customer.

We require credit card for first purchase. PO’s can be used once accounts are setup.

Formal quotes available, please inquire.

Stain Procedure

Gormori’s One Step Trichrome

  1. De-paraffin slides in xylene –  2 minutes
  2. De-paraffin slides in xylene – 2 minutes
  3. Clear slides in 100% alcohol – 2 minutes
  4. Clear slides in 100% alcohol – 2 minutes
  5. Hydrate slides in 95% alcohol – 2 minutes
  6. Hydrate slides in running water – 5 minutes
  7. Bouins at 60 degrees Celsius – 1 hour
  8. Wash in running water – 5 minutes
  9. Weigert’s Hematoxylin (make fresh)   5-10 minutes  
  10. Wash in running water  5-7 minutes
  11. Stain in Gomori’s Trichrome solution for 15-20 minutes
  12. Acetic Acid solution – 1 minutes
  13. Wash distilled water – 10 seconds
  14. Dehydrate in 95% alcohol – 1 minute
  15. Dehydrate in 100% alcohol – 1 minute
  16. Dehydrate in 100% alcohol – 1 minute
  17. Clear in xylene – 1 minute
  18. Clear in xylene – 2 minutes

Results:

Nuclei- Black

Cytoplasm, keratin, muscle fiber and intercellular fibers- Red

Collagen – Blue

Contact us

anytime at:

JMD Histology & Histologistics Inc.

508-461-7207

admin@jmdhistology.com

Ordering Information