Histology Services

We offer a wide array of histology services and turnaround times to fit your needs. Both paraffin and cryogenic tissue services available for every type of tissue. Cell pellets, decalcification, gross dissection, no problem. Tissue micro arrays (TMA) can be made with paraffin and frozen tissues with different size cores for your projects. Antibody workups and staining is another specialty of ours. We have human normal and abnormal, normal mouse and rat tissues for controls. Over 50 working antibody protocols to choose from.

PARAFFIN PROCESSING

We accept tissues in cassettes or ones that need to be cassetted. They are processed on 1 of 2 fully automated tissue processors.  After processing, we embed your tissues in fresh high quality paraffin.

CRYOGENIC PROCESSING

Send us your fresh, formalin fixed or oct embedded tissues for cryostat cutting. Choose an array of formats for slides to be routine, special, IHC, or IF stained. Slides can also be fixed or not, left unstained and sent back to you for your staining. Select any thickness you want, we can do it.

Tissue microarrays(TMA) can be made and cut using your frozen tissues. Please inquire.

We have Bregma brain map knowledge to section specific areas of the brain. Please call to arrange a conversation with a brain cutting specialist.

PARAFFIN SECTIONING

Our paraffin cutting experience is unmatched. Over the years we have received and cut more types of human, animal, marine, insect, lizard, avian, amphibian species than most other labs have ever seen. Give us your toughest tissues to cut and stain. Our techs and microtomes can handle anything you throw at us.

We focus on quality sections not speed. Blocks are trimmed so that at least 95% of the tissue in the block is visible on the slide. Imperfections like, bubbles, tears, and wrinkles are frowned upon by our QC department.

TISSUE MICROARRAY (TMA)

In many tissue mircoarrays the cores float around in the waterbath. When this happens, the core orientation can change resulting in stained slides that are difficult to read. Our TMA making process is better. Cores are surrounded by liquid paraffin rather than embedded in pre-made blocks. This process does not allow core orientation to change ever.

Both paraffin and fresh (frozen) tissue services are available for making TMA blocks from your existing tissues. We use a new system for making TMA blocks that is less time-consuming and does not use the traditional tools and equipment.  This allows us to charge less and offer faster turnaround times. 

MICROSCOPIC & SCANNING PICTURES

Do you need high quality micrographs for your publications? We have several microscopes with cameras that can take 4x, 10x, 20x, 40x and oil pictures. If you look at our pictures found here https://histologistics.com/histology/histology-microscopic-pictures/, we have taken all these plus tons more.

Do you want high resolution scans that can be used for your papers or cellular analysis? Then we have the solution. We offer scanning at 20x and 40x for light microscopy.

ROUTINE & SPECIAL STAINING

Slide staining is a very important part of the histology process. No matter what you are looking for H&E staining is essential for general morphology. Every published paper has a least 1 H&E picture. We use our own stain formula that gives nuclei a vibrant purple and other tissues 3 shades of pink.

For special stains, we use the most consistent protocols like, Masson’s trichrome, Gomori’s basement membrane, Snook’s reticulin, Mcmanus PAS, Grocott’s (GMS). All stains are QC’d with our positive control slides that are also given to you.

IMMUNOHISTOCHEMISTRY & IMMUNOFLUORESCENCE

Do you have a desired target protein? We have the solution. Antibodies can target everything, proteins, bacteria, virus, fungus and prions. No matter what your target is, we can help you see it.

We can get any antibody to work using our TMA’s and other control tissues. More than 50 working antibody protocols have been done for previous customers.