Human Appendix Ki67

IHC / IF Antibodies Staining

Hello and welcome to our immunohistochemistry and immunofluorescence staining services.

This can be a difficult but very effective tool for visualizing your target epitopes. Our technicians can help decipher your antibody target.

Let us help your research fulfill its greatest potential with antibody markers of your choice.
In our experience, antibodies are a valuable tool that can make or break your research. Proteins can be finicky, and stubborn, and sometimes the workups are tough but in the end most (99%) of them work. That’s why we’re here, to do the difficult jobs nobody wants to do.

New antibodies are being tested for both customers (their targets) and ourselves.  If you do not see a desired target, please inquire. One positive and one negative control is used for each client project and sent back with the slides. Isotype controls can be also done if desired and supplied by you.

We routinely work with Human, Mouse and Rat tissues but also have experience with canine, feline, horse, porcine and toad species for IHC staining.

Most of our antibody stains are initially done by hand but then, if enough there is volume and repeat slides, we convert the procedure onto our Dako automated system. This decreases turnaround times and staining variability between slides.

IHC & IF performed by us is usually done by the customer buying the antibody and send it to us. We provide secondary antibodies and all other necessary reagents. Positive control slides are the responsibility of the researcher. Our lab does have some positive controls which can be bought, please inquire.

We know the primary antibody usually cost the most but the secondary antibodies we purchase, from Vector Labs, also costs about the same amount. Providing all the other reagents is also our part. We know which chemicals work best with respective antibodies.

There are 100 plus antibodies that we have worked up and now have consistent protocols for. Most are antibodies monoclonals, we prefer to work with them but some are polyclonals.

All antibodies have data sheets from their respective manufacturers, contact us if desired.

Our experience speaks for itself. Take a look at our microscopic pictures, these are all antibodies stained by us. Tissues are positive controls and photographed in our lab.

2 thoughts on “IHC / IF Antibodies Staining

  1. Hi Hans. The tonsil controls we you made for us here at Borsting’s Labs were excellent! The sections were perfect and the chromogen “lit up” beautifully with immunoperoxidase staining.

  2. Hi Jeff,

    I’m glad everything went so well. I stained one slide with H&E just to see if everything was in the right place. Let us know if you need anything else!

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