Antibodies

In-House Antibodies

We are constantly testing new antibodies both for customers (their targets) and ourselves.  If you do not see a desired target, please inquire.  As for favorite antibody manufactures, we use a wide variety, but prefer ones that work on the first try.  Most antibodies are intended for paraffin HRP but we have gotten a few to work for fluorescent as well.  If possible, we generally choose monoclonal since they are more specific and generally produce less background staining.  Using our staining techniques, we use very little antibody per slide, usually 50ul.  This is a huge savings for you the client as we can hold your antibodies at our facility and stain large numbers of slides for you.

Our in house antibodies listed in the pricing pdf (p18). https://histologistics.com/histology-service/services/pricing-list/.  They have been worked up by us and usually target more than one species.  Most of them are tested using at least 2 species both normal and abnormal tissues if possible.  We have numerous pictures, some posted here,  on various tissue types.  All antibodies have data sheets from their respective manufacturers, contact us if desired.

Antibodies Listed / Manufacturer Links / Pictures

Anti-neutrophil antibody (ANA) – Abcam ab2557– Rat monoclonal, Target – Mouse.

 

Beta-Catenin (CTTNB1) – Biolegend 844602 – Recombinant, Targets-Human, Mouse, Rat.

Caspase 3 – Santa Cruz – Santa Cruz – Caspase 3 – Mouse monoclonal – Target – Human

CD3 – NovusBio – Novusbio CD3 – Polyclonal – Targets – Human, canine, equine, feline, primate.  In house testing on canine and human.

CD8 – Invitrogen MA5-14373 – Mouse monoclonal – Targets – Mouse, rat, human.

CD11b – Abcam ab133357 – Rabbit monoclonal – Targets – mouse, human.

CD11b antibody
Human tumor CD11b

CD15 – Abcam ab188610 – Mouse monoclonal – Target – Human.

Spleen IHC

Human spleen CD15

CD31 – Abcam ab28364 – Rabbit polyclonal – Targets – Human, mouse, pig.

CD8 antibody
Tumor CD31 40x

CD20 –Biocare CD20 – Rabbit polyclonal – Targets – Human, canine, feline, horse, cow, pig, sheep.  In house testing in Canine and Human.

CD45 – Abcam ab10558 – Rabbit polyclonal – Targets – Mouse, rat, human, pig, rhesus monkey.

CD45 – Santa Cruz – CD45 – Mouse monoclonal – Targets – Human, Mouse, Rat.

CD68 – Abcam – ab955 – Mouse monoclonal – Targets – mouse, human, rat, rabbit.

Ck20 – Santa Cruz – sc-271183 – Mouse Monoclonal – Targets – Human, Mouse, Rat.  In house applications tested – Human, Mouse, and Rat.

Cytomegalovirus – NovusBio NBP1-22532 – Mouse monoclonal – Target – Virus

F4/80 – Santa Cruz sc-377009 – Mouse monoclonal – Targets – Mouse, rat, human.

FOXP3 – Santa Cruz sc-53876 – Mouse monoclonal – Targets – Mouse, rat, human.

lymphoma IHC
Human lymphoma FoxP3

Glucagon (GLP1) –  Santa-Cruz – sc-57166 – Mouse Monoclonal – Targets – Human, Mouse, Rat.  Tested applications – Human, Mouse and Rat.

GLUT2 – Santa Cruz – sc-518022 – Mouse Monoclonal – Targets – Human, Mouse, Rat.  Tested applications – Mouse

Granzyme B – Santa Cruz sc-8022 – Mouse Monoclonal – Targets – Human, Mouse, Rat

Helicobacter Pylori – Scytek RA0379-C.5-IFU-RUOrev1, Rabbit polyclonal – Target – H. pylori bacterium.

HMGB1 – Abcam – 79823– Rabbit monoclonal – Targets – Mouse, rat, human.

IGF1R – Bioss – 0227R – Rabbit polyclonal – Targets – Human, mouse, rat.

Indoleamine 2,3-dioxygenase (IDO) – Santa Cruz -53978 – Rat monoclonal – Targets – Mouse, rat, human.

Insulin – Santa Cruz Insulin – Mouse monoclonal – Targets – Human, Mouse, Rat.  Tested applications – Mouse

Kappa – Santa Cruz – Kappa –  Mouse Monoclonal – Target – Human

Ki-67 – Abcam 16667 – Rabbit monoclonal – Targets – Mouse, rat, human, common marmoset.

Lambda – Santa Cruz – Lambda – Mouse monoclonal – Target – Human

Ly-6B.2 – Bio-rad MCA771GA – Mouse monoclonal – Target – Mouse.

Mouse spleen IHC
Mouse spleen Ly6b.2

Ly-6G (GR1) – Biolegend -108436 – Rat monoclonal – Target – Mouse.

Melanoma Marker (HMB45) – Santa Cruz – HMB45 – Mouse monoclonal – Targets – Human, Mouse, Rat.

Pan-Cytokeratin – (AE1/AE3) – Santa Cruz – 81714 – Mouse monoclonal – Targets – Human, mouse, rat.

Perforin – Santa Cruz – 136994 – Mouse monoclonal – Targets – Mouse, rat, human.

S100 – Santa Cruz – S100 – Mouse monoclonal – Targets – Mouse, Rat, Human.  In house testing done one Mouse and human.

Smooth Muscle Actin (1A4) – Abcam 7817 – Mouse monoclonal – Targets – Mouse, rat, sheep, rabbit, human, pig.

Synaptophysin – Santa Cruz – Synaptophysin  – Mouse monoclonal – Targets Human, mouse, rat.  In house testing done on Human, mouse and rat.

DeadEnd Tunel – Apoptosis – Promega – Tunel

Vimentin – Santa Cruz – Vimentin – Mouse Monoclonal – Targets – Human, Mouse, Rat.

Yap (yes associated protein) – Santa Cruz – YAP – Mouse monoclonal – Targets – Human, mouse, rat.  In house testing done on human, mouse and rat.

2 thoughts on “Antibodies

  1. Hi Hans. The tonsil controls we you made for us here at Borsting’s Labs were excellent! The sections were perfect and the chromogen “lit up” beautifully with immunoperoxidase staining.

  2. Hi Jeff,

    I’m glad everything went so well. I stained one slide with H&E just to see if everything was in the right place. Let us know if you need anything else!

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