Antibodies

In-House Antibodies

We are constantly testing new antibodies both for customers (their targets) and ourselves.  If you do not see a desired target, please inquire.  As for favorite antibody manufactures, we use a wide variety, but prefer ones that work on the first try.  Most antibodies are intended for paraffin HRP but we have gotten a few to work for fluorescent as well.  If possible, we generally choose monoclonal since they are more specific and generally produce less background staining.  Using our staining techniques, we use very little antibody per slide, usually 50ul.  This is a huge savings for you the client as we can hold your antibodies at our facility and stain large numbers of slides for you.

 

Antibody List

ANA  F4/80  SMA (1A4)
Beta-catenin  FOXP3  Synaptophysin
CD3  Granzyme B  YAP  Tunel
CD8  Helicobacter Pylori  CEA  Insulin
CD11b  HMGB1  Kappa  Glut2
CD15  IGF1R  Lambda  Glucagon (GLP1)
CD20  Indolamine  PSA
CD31  KI67  S100
CD45  Ly-6B.2  Vimentin
CD68  Ly-6G  HMB45
CK20  Pan Keratin (AE1/AE3)
CMV  Perforin  Caspase 3

 

These our in house antibodies.  They have been worked up by us and usually target more than one species.  Most of them are tested using at least 2 species both normal and abnormal tissues if possible.  We have numerous pictures, some posted here,  on various tissue types.  All antibodies have data sheets from their respective manufactures, contact us if desired.

Antibodies Listed / Manufacturer Links / Pictures

Anti-neutrophil antibody (ANA) – Abcam ab2557– Rat monoclonal, Target – Mouse.

Mouse bone marrow ANA 10x
Mouse bone marrow ANA 10x
Mouse bone marrow ANA 10x

 

 

 

 

 

 

Beta-Catenin (CTTNB1) – Biolegend 844602 – Recombinant, Targets-Human, Mouse, Rat.

 

Caspase 3 – Santa Cruz – Santa Cruz – Caspase 3 – Mouse monoclonal – Target – Human

 

CD3 – NovusBio – Novusbio CD3 – Polyclonal – Targets – Human, canine, equine, feline, primate.  In house testing on canine and human.

 

CD8 – Invitrogen MA5-14373 – Mouse monoclonal – Targets – Mouse, rat, human.

CD11b – Abcam ab133357 – Rabbit monoclonal – Targets – mouse, human.

CD11b antibody
Human tumor CD11b

 

 

CD15 – Abcam ab188610 – Mouse monoclonal – Target – Human.

Spleen IHC
Human spleen CD15

 

CD31 – Abcam ab28364 – Rabbit polyclonal – Targets – Human, mouse, pig.

CD8 antibody
Tumor CD31 40x

 

 

CD20 –Biocare CD20 – Rabbit polyclonal – Targets – Human, canine, feline, horse, cow, pig, sheep.  In house testing in Canine and Human.

 

CD45 – Abcam ab10558 – Rabbit polyclonal – Targets – Mouse, rat, human, pig, rhesus monkey.

CD45 – Santa Cruz – CD45 – Mouse monoclonal – Targets – Human, Mouse, Rat.

 

CD68 – Abcam – ab955 – Mouse monoclonal – Targets – mouse, human, rat, rabbit.

 

Ck20 – Santa Cruz – sc-271183 – Mouse Monoclonal – Targets – Human, Mouse, Rat.  In house applications tested – Human, Mouse, and Rat.

 

Cytomegalovirus – NovusBio NBP1-22532 – Mouse monoclonal – Target – Virus

 

F4/80 – Santa Cruz sc-377009 – Mouse monoclonal – Targets – Mouse, rat, human.

 

FOXP3 – Santa Cruz sc-53876 – Mouse monoclonal – Targets – Mouse, rat, human.

lymphoma IHC
Human lymphoma FoxP3

 

Glucagon (GLP1) –  Santa-Cruz – sc-57166 – Mouse Monoclonal – Targets – Human, Mouse, Rat.  Tested applications – Human, Mouse and Rat.

 

 

GLUT2 – Santa Cruz – sc-518022 – Mouse Monoclonal – Targets – Human, Mouse, Rat.  Tested applications – Mouse

 

Granzyme B – Santa Cruz sc-8022 – Mouse Monoclonal – Targets – Human, Mouse, Rat

 

Helicobacter Pylori – Scytek RA0379-C.5-IFU-RUOrev1, Rabbit polyclonal – Target – H. pylori bacterium.

 

HMGB1 – Abcam – 79823– Rabbit monoclonal – Targets – Mouse, rat, human.

 

IGF1R – Bioss – 0227R – Rabbit polyclonal – Targets – Human, mouse, rat.

Indoleamine 2,3-dioxygenase (IDO) – Santa Cruz -53978 – Rat monoclonal – Targets – Mouse, rat, human.

 

Insulin – Santa Cruz Insulin – Mouse monoclonal – Targets – Human, Mouse, Rat.  Tested applications – Mouse

 

Kappa – Santa Cruz – Kappa –  Mouse Monoclonal – Target – Human

 

Ki-67 – Abcam 16667 – Rabbit monoclonal – Targets – Mouse, rat, human, common marmoset.

 

Lambda – Santa Cruz – Lambda – Mouse monoclonal – Target – Human

 

Ly-6B.2 – Bio-rad MCA771GA – Mouse monoclonal – Target – Mouse.

Mouse spleen IHC
Mouse spleen Ly6b.2

 

Ly-6G (GR1) – Biolegend -108436 – Rat monoclonal – Target – Mouse.

 

Melanoma Marker (HMB45) – Santa Cruz – HMB45 – Mouse monoclonal – Targets – Human, Mouse, Rat.

 

 

Pan-Cytokeratin – (AE1/AE3) – Santa Cruz – 81714 – Mouse monoclonal – Targets – Human, mouse, rat.

Perforin – Santa Cruz – 136994 – Mouse monoclonal – Targets – Mouse, rat, human.

 

S100 – Santa Cruz – S100 – Mouse monoclonal – Targets – Mouse, Rat, Human.  In house testing done one Mouse and human.

Smooth Muscle Actin (1A4) – Abcam 7817 – Mouse monoclonal – Targets – Mouse, rat, sheep, rabbit, human, pig.

 

Synaptophysin – Santa Cruz – Synaptophysin  – Mouse monoclonal – Targets Human, mouse, rat.  In house testing done on Human, mouse and rat.

 

DeadEnd Tunel – Apoptosis – Promega – Tunel

 

Vimentin – Santa Cruz – Vimentin – Mouse Monoclonal – Targets – Human, Mouse, Rat.

 

Yap (yes associated protein) – Santa Cruz – YAP – Mouse monoclonal – Targets – Human, mouse, rat.  In house testing done on human, mouse and rat.

2 thoughts on “Antibodies

  1. Hi Hans. The tonsil controls we you made for us here at Borsting’s Labs were excellent! The sections were perfect and the chromogen “lit up” beautifully with immunoperoxidase staining.

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  2. Hi Jeff,

    I’m glad everything went so well. I stained one slide with H&E just to see if everything was in the right place. Let us know if you need anything else!

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