Vimentin Antibody Protocol


This antibody is from Santa Cruz Biotechnology and can be found here sc-55507.

This is a very easy antibody stain that works extremely well on human tissues but a little more difficult on mouse tissues. If you are working with mouse tissues, I suggest using heat and longer incubation time during the primary antibody step. Mouse and I suspect rat tissues are more difficult to get a good signal compared to human.

As with many of the other mouse monoclonal antibodies, staining mouse tissues may need additional blocking to reduce unwanted background staining.

As always, when testing this and all new protocols, be sure to use your controls first to view staining results. The protocol may need to be tweaked to your tissues.

Human Colon Vimentin antibody stain