Melanoma Marker (HMB45) Antibody Protocol


This antibody can be found on the Santa Cruz Biotechnology website sc-59305.

Antibody was tested in 2017 with mouse skin and human melanoma, tissues.

A relatively easy stain. Make sure the tris buffer is at pH9.0 and use a less harsh cook setting. Note: Heating the slides to 60C during primary and secondary incubation helps the stain come in dark.

Human skin (melanoma) stained with HMB45 antibody