This antibody is from Cell Signal Technology PECAM-1 (89C2) and can be found here CST-3528.

This is a very easy antibody stain that works very well on human tissues.

As always, when testing this and all new protocols, be sure to use your controls first to view staining results. The protocol may need to be tweaked to your tissues.

This antibody is from Abcam and can be found here ab28364.

I had trouble with antibody stain. Not sure why though, it could have been a rush project and we just didn’t have time to work it out properly. The results were just ok.

The Abcam antibodies are great, at least 30% of the protocols here use Abcam antibodies. The Abcam website, I find, is very easy to use, partial protocols can be found throughout.

As always, when testing this and all new protocols, be sure to use your controls first to view staining results. The protocol may need to be tweaked to your tissues.