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The first antibody protocol (Novus) I worked up with human and canine tissues and it works great. It does not however target mouse tissue.
This antibody can be found on the Novus Biological website NB600-1441SS.
Antibody was tested in 2019 with human tonsil, thymus or canine lymph tissues.
The freshly cut controls are helpful.
I do however have some help with another CD3 antibody (Abcam ab16667) that does target mouse tissues (see data sheet below). A partner lab Histologistics worked with used the following steps on mouse tissues. Hint: Mouse spleen as positive control. Dilution 1:150. HIER – Sodium citrate pH 6.0 for 30 minutes. This is a rabbit monoclonal so be sure to use an anti-rabbit secondary.