Anti-neutrophil Antibody (ANA) protocol


This is Abcam’s (NIMP-14) ab2557. A mouse monoclonal antibody. Even though the data sheet states IHC frozen, I believe I got this to work for IHC paraffin. The bone marrow picture shows a silver DAB with with nuclear fast red background stain. Any DAB color would work.

Later on I did Ly-6G antibody on both frozen and paraffin tissues.