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Amyloid (Congo Red) Tissue Control Slides On Sale $1.37 Per Slide

Human Kidney Amyloid Congo red 1

 

This is an incredible tissue control sale that will only last 30 days.  We have high quality amyloid for congo red staining control tissues for just $1.37 per slide!

These tissues are placed on positively charged slides and baked for 60 minutes to ensure their adhesion.  All slides are cut to order and can be customized to meet your needs.  We can place negative control tissue right next to the amyloid tissue and orient to your specifications.

Orders will be taken be email at a first come first serve basis.  No order is considered too small or too large, if we still have the tissue you will get it.

Most other histology supply companies charge between $3.15 to $6.00 per slide.  This is a 1 month sale and will expire on 8/24/2014 or when the tissue is gone.

Please contact us for a sample. (US only)

Histologistics Inc.
Hans B Snyder
60 Prescott Street
Worcester, MA 01605
hans@histologistics.com

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Congo Red Stain Protocol For Amyloid

Run a control slide.                                     Control tissue – Tissue with amyloid

Safety equipment: Work under a hood with lab coat, gloves, and glasses.

  1. De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
  2. De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
  3. Clear slides in 100% alcohol———————- 2 minutes
  4. Clear slides in 100% alcohol———————- 2 minutes
  5. Hydrate slides in 95% alcohol——————– 2 minutes
  6. Hydrate slides in running water —————– 5 minutes
  7. 1% Congo red ————————————— 60 minutes (re-use)
  8. Rinse in water ————————————— 1 minute
  9. Alkaline Alcohol ————————————- until background clear (re-use)
  10. Wash in running water —————————- 5 minutes
  11. Harris Hematoxylin ——————————— 30 seconds (re-use)
  12. Wash in running water —————————- 10 minutes
  13. Dehydrate in 95% alcohol———————— 30 seconds
  14. Dehydrate in 100% alcohol——————— 1 minute
  15. Dehydrate in 100% alcohol——————— 2 minutes
  16. Dehydrate in 100% alcohol——————— 2 minutes
  17. Clear in xylene (3)——————————– 2 minutes (re-use)
  18. Clear in xylene (4)——————————– 5 minutes (re-use)
  19. Coverslip

Results:         Amyloid – Pink to red                     Nuclei – Blue

With polarizing microscope

Amyloid – Green Birefringence

 

An Expert Histology Service