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Customized Histology Staining for Your Projects

 

Masson’s Trichrome blue and green in the same tissues:

Blue                                     Green                                    Blue                                           Green

Histology slide pictures of Trichrome blue vs green

 

We have over 40 special stain protocols that can be customized to fit your needs.  We realize that not every histology lab’s stains look the same, that’s why we are will to change, and modify our protocols to suit your project.  The subtle differences of one stain, in our eyes may be minute but what counts is our clients preferences.

In the past, we have done custom stains such as modifying the trichrome blue to a green.  Looking at them in the colon, the colors are very similar but the umbilical cord has marked changes.  Striving to meet your needs not matter the application, we are all about your needs.  This goes beyond staining, we will change any of our histology services (protocols) to make a good fit for you.

 

Call us for more information.

Hans Snyder – 508-308-7800

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Histology Stain Picro-sirius Red With Nuclei Stain

 

A comparison of the picrosirius red stain with and without a nuclear stain.

We took the original picrosirius red stain and modified it to create our version with crisp looking nuclei.

The picture on the left has been stained with Weigert’s hematoxylin and on the right, no nuclear stain.

Collagen – Red         Muscle – Green       Nuclei – black

 

histology stain picro-sirius red

What is your preference?

Collagen – the tissue glue, plays a vital role in maintaining structural integrity in tissue function.  This stain is used to detect many disease models such as liver fibrosis, skin wounds, myocardial scars, and arterial tissue.

 

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Verhoeff Van Gieson Stain

Safety equipment: Work under a hood with lab coat, gloves, and glasses.

  1. De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
  2. De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
  3. Clear slides in 100% alcohol———————- 2 minutes
  4. Clear slides in 100% alcohol———————- 2 minutes
  5. Hydrate slides in 95% alcohol——————— 2 minutes
  6. Hydrate slides in running water——————- 5 minutes
  7.  VVG working solution—————————— 60 minutes (re-use)
  8. Wash in running water—————————— 2 minutes
  9. Differentiate sections microscopically in 2% ferric chloride until the elastic fibers are distinct and the background is colorless to light grey.  If the sections are differentiated too far, re-stain. (see notes below)
  10. Rinse in DH2O—————————————- 1 minute
  11. Sodium thiosulfate———————————– 1 minute (re-use)
  12. Wash in running water—————————— 5 minutes
  13. Counterstain in Van Gieson’ s——————— 1 minute (re-use)
  14. Dehydrate in 95% alcohol————————– 1 minute
  15. Dehydrate in 95% alcohol———————— 1 minute
  16. Dehydrate in 100% alcohol———————- 1 minute
  17. Dehydrate in 100% alcohol———————- 2 minutes
  18. Dehydrate in 100% alcohol———————- 2 minutes
  19. Clear in xylene (3)———————————- 2 minutes
  20. Clear in xylene (4)———————————- 5 minutes
  21. Coverslip 

Results:

Elastic Fibers —————————————- Black

Background —————————————— Yellow

Notes:

  1. It is easy to over differentiate.  If the background is completely colorless so that a clear yellow counterstain is obtained, the section may be over differentiated.  It is probably better to err on the side of under differentiating.
  2. Over differentiated sections can be re-stained.
  3. Do not prolong the Van Gieson stain; picric acid could differentiate the stain further.
  4. Be sure to follow the order of adding all reagents when making the Verhoeff’s solution.
  5. To clean the Verhoeff’s stain out of the jar, wash with 2% ferric chloride solution for a few minutes.
  6. For optimal results differentiate the control slide first.

Porcine Aorta VVG 40x

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Special Stain Solution Protocols Page

Hello,  We have starting adding our solution protocols on a new page.  These are all the protocols that we use now. A professional histology service.

http://histologistics.com/histology/special-stain-solution-protocols/

An Expert Histology Service