Category: histopathology

pathology reports

Colon Cancer

Colon Adenocarcinoma

by Histology Reference Guide, posted in histology service, histopathology, IHC

Pathology of colon cancer as seen through the eyes of a histologist.  How many different ways are there to visualize cancer?  The more the better!

Hematoxylin & Eosin (our recipe)

Human colon adenocarcinoma H&E

HMGB1 Antibody – According to the The Human Protein Atlas HMGB1 protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration.

Human colon adenocarcinoma HMGB1

CD8 Antibody in the Human Protein Atlas ( CD8A) describes it as a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.

Human colon adenocarcinoma CD8

Indoleamine Antibody – This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity (IDO1).

Human Colon adenocarcinoma IDO1
Human Colon adenocarcinoma IDO1

Perforin Antibody – This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis (Perforin).

Colon cancer
Colon Adenocarcinoma Perforin Antibody

 

Note: All tissues were bought, cut and stained at Histologistics Inc as positive control slides.  These are not part of any study.

Human skin (melanoma?) H&E

Melanin or Melanoma?

by Histology Reference Guide, posted in FFPE tissues, H&E, Histologistics, Histology, histology service, histopathology, IHC

Melanoma, a heightened subject that should be talked about with, everyone?  How do you tell the difference between melanin and melanoma?  Research facilities are currently exploring this topic and it’s treatment.  But we do know the primary cause, too much UV rays.  The pathologist can see the difference between melanin and melanoma an H&E stained section, can you?.  Here are some examples.

Human Skin H&E with melanin
Human Skin H&E with melanin
Human skin (I think) stained with H&E
Human skin (I think) stained with H&E 40x

For the rest of us, melanoma is better detected by antibody staining (IHC).  There are a few different antibodies, MART1, Melan, HMB but all I have is Granzyme B.

Human skin Granzyme B 10x Possible melanoma
Human skin Granzyme B 40x Possible melanoma

What do you think?

Ovarian Carcinoma

by Histology Reference Guide, posted in FFPE tissues, H&E, Histologistics, Histology, histology service, histopathology

In this tissue, it’s diagnosis is ovarian carcinoma.  It’s stained with H&E and pictures taken at multiple areas.  Some (not posted here) look to have melanin deposits but that will have to be discovered using different staining techniques and will update again here.

Enjoy!

Ovarian Carcinoma H&E

What Kind of Fungus is This?

by Histology Reference Guide, posted in histopathology

You know there’s something wrong with the GMS stain when everything turns black.  Time to make new reagents.  After they are all made, it’s right to test the stain with known control tissue.  After the staining was complete, a variety of black components covered my slides.  The GMS stain is a silver stain that fungi “reduce” and then appear black.  Unfortunately other tissues also can turn black and add confusion to the diagnosis of a type of fungus.  After the slides were dried for 24 hours, I took high-resolution pictures of the black substances in my tissues to hopefully get help in fungi identification from you.  Below are 6 different slides with possible fungi.  If anyone knows what any of these pictures could be please comment below.  All comments are welcome.

  1. Muc 1.1 GMS 20x

 

 

 

 

 

 

 

 

 

 

2. Fungus 2 GMS 100x

 

 

 

 

 

 

 

 

 

 

 

 

3.

FUN 6 GMS 100x

 

 

 

 

 

 

 

 

 

 

 

 

4.

Fun 1.1 GMS 100x

 

 

 

 

 

 

 

 

 

 

 

 

5.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

FUN GMS 2.1 100x

 

 

 

 

 

 

 

 

 

 

 

 

6. GMS 1.1 100x