Category: histopathology

pathology reports

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Colon Adenocarcinoma

Colon Cancer

Pathology of colon cancer as seen through the eyes of a histologist.  How many different ways are there to visualize cancer?  The more the better!

Hematoxylin & Eosin (our recipe)

Human colon adenocarcinoma H&E

HMGB1 Antibody – According to the The Human Protein Atlas HMGB1 protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration.

Human colon adenocarcinoma HMGB1

CD8 Antibody in the Human Protein Atlas ( CD8A) describes it as a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.

Human colon adenocarcinoma CD8

Indoleamine Antibody – This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity (IDO1).

Human Colon adenocarcinoma IDO1
Human Colon adenocarcinoma IDO1

Perforin Antibody – This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis (Perforin).

Colon cancer
Colon Adenocarcinoma Perforin Antibody

 

Note: All tissues were bought, cut and stained at Histologistics Inc as positive control slides.  These are not part of any study.

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Melanin or Melanoma?

Human skin (melanoma?) H&E

Melanoma, a heightened subject that should be talked about with, everyone?  How do you tell the difference between melanin and melanoma?  Research facilities are currently exploring this topic and it’s treatment.  But we do know the primary cause, too much UV rays.  The pathologist can see the difference between melanin and melanoma an H&E stained section, can you?.  Here are some examples.

Human Skin H&E with melanin
Human Skin H&E with melanin
Human skin (I think) stained with H&E
Human skin (I think) stained with H&E 40x

For the rest of us, melanoma is better detected by antibody staining (IHC).  There are a few different antibodies, MART1, Melan, HMB but all I have is Granzyme B.

Human skin Granzyme B 10x Possible melanoma
Human skin Granzyme B 40x Possible melanoma

What do you think?

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Ovarian Carcinoma

In this tissue, it’s diagnosis is ovarian carcinoma.  It’s stained with H&E and pictures taken at multiple areas.  Some (not posted here) look to have melanin deposits but that will have to be discovered using different staining techniques and will update again here.

Enjoy!

Ovarian Carcinoma H&E
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Helicobacter Pylori Tissue Control Slides On Sale $1.37 Per Slide (IHC Only)

Human Colon IHC Helicobacter Pylori
Human Colon IHC Helicobacter Pylori

This is an incredible tissue control sale that will only last 30 days.  We have high quality helicobacter pylori (HP) tissue for IHC staining control tissues for just $1.37 per slide!  This tissue is a little different from normal (HP) because it’s from the lower GI tract.  Some pieces contain small intestine and some are colon.  These controls have been bought by area hospitals and have been in use for over a year.   These have been tested with special stains such as Giemsa, warthin starry and Steiner and Steiner and are not suitable for these tests.

These tissues are placed on positively charged slides that can be baked at 60C for 1 hour or overnight at 37C for to make sure their adhesion.  All slides are cut to order and can be customized to meet your needs.  We can place negative control tissue right next to the positive tissue and orient to your specifications.

Orders will be taken be email at a first come first serve basis.  No order is considered too small or too large, if we still have the tissue you will get it.  We ship worldwide.

Most other histology supply companies charge between $3.15 to $6.00 per slide.  This is a 1 month sale and will expire on 9/07/2014 or when the tissue is gone.

Please contact us for a sample. (US only)

Histologistics Inc.
Hans B Snyder
60 Prescott Street
Worcester, MA 01605
hans@histologistics.com