Melanoma, a heightened subject that should be talked about with, everyone? How do you tell the difference between melanin and melanoma? Research facilities are currently exploring this topic and it’s treatment. But we do know the primary cause, too much UV rays. The pathologist can see the difference between melanin and melanoma an H&E stained section, can you?. Here are some examples.
Human Skin H&E with melanin
Human Skin H&E with melanin
Human skin (I think) stained with H&E
Human skin (I think) stained with H&E 40x
For the rest of us, melanoma is better detected by antibody staining (IHC). There are a few different antibodies, MART1, Melan, HMB but all I have is Granzyme B.
In this tissue, it’s diagnosis is ovarian carcinoma. It’s stained with H&E and pictures taken at multiple areas. Some (not posted here) look to have melanin deposits but that will have to be discovered using different staining techniques and will update again here.
For us in histology, normal tissue identification is typically an easy process. As histologists, we were once required to identify all normal tissues by H&E under the microscope.
For abnormal tissue however, an H&E stained slide can be very difficult to identify tissue type and diagnosis. That’s why when a pathologist orders multiple stains on one tissue, they are trying to get a larger picture to understand its etiology.
To help in understanding, here are 3 stains on the same tissue, H&E, SMA, & CD8 at 4x & 10x. This is human tissue. Can you identify this tissue and its diagnosis?
This is an incredible tissue control sale that will only last 30 days. We have high quality helicobacter pylori (HP) tissue for IHC staining control tissues for just $1.37 per slide! This tissue is a little different from normal (HP) because it’s from the lower GI tract. Some pieces contain small intestine and some are colon. These controls have been bought by area hospitals and have been in use for over a year. These have been tested with special stains such as Giemsa, warthin starry and Steiner and Steiner and are not suitable for these tests.
These tissues are placed on positively charged slides that can be baked at 60C for 1 hour or overnight at 37C for to make sure their adhesion. All slides are cut to order and can be customized to meet your needs. We can place negative control tissue right next to the positive tissue and orient to your specifications.
Orders will be taken be email at a first come first serve basis. No order is considered too small or too large, if we still have the tissue you will get it. We ship worldwide.
Most other histology supply companies charge between $3.15 to $6.00 per slide. This is a 1 month sale and will expire on 9/07/2014 or when the tissue is gone.
Please contact us for a sample. (US only)
Hans B Snyder
60 Prescott Street
Worcester, MA 01605