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Abnormal Tissue Identification

For us in histology, normal tissue identification is typically an easy process.  As histologists, we were once required to identify all normal tissues by H&E under the microscope.

For abnormal tissue however, an H&E stained slide can be very difficult to identify tissue type and diagnosis.  That’s why when a pathologist orders multiple stains on one tissue, they are trying to get a larger picture to understand its etiology.

To help in understanding, here are 3 stains on the same tissue, H&E, SMA, & CD8 at 4x & 10x.  This is human tissue.  Can you identify this tissue and its diagnosis?

 

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I’ll give you choices.

A. Lung – Spindle cell carcinoma

B. Liver – Cirrhosis

C. Colon – Adenocarcinoma

D. Heart – Myocardial fibrosis

 

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Fungus FFPE Blocks and Slides for Sale

Fungus blocks for sale

We have a number of extra FFPE human peritoneum tissues with fusarium, pseudallescheria boydii and or aspergillus fungus infection.  They have been tested by us and some work well for both GMS and PAS.  There is fungus throughout the tissue.

Human Peritoneum Fungus GMS
Human Peritoneum Fungus GMS

If you need high quality, easy to read, human fungus unstained control slides, we can make that happen! Once blocks are purchased, we can cut the blocks down and or makes slides to your specifications.  Why would you pay $3.50 to $8.00 per slide when you can get them for so much less here? Fungus Control FFPE Blocks and Slides

Human Peritoneum Fungus PAS
Human Peritoneum Fungus PAS

We are a full service histopathology lab that has been in business for 5+ years.  With our histology experience, we provide paraffin, cryogenic, plastic, TMA’s, routine, special and IHC/IF staining.  Being such, we can cut one or more slides and stain 45 different ways, for your review at additional cost.

Human Peritoneum Fungus GMS
Human Peritoneum Fungus GMS

 

Buy now and Save$$$

Block price – $95.00

Unstained slides on positively charged slides – $1.50 per slide (minimum 100)

GMS and PAS stained slides – $9.00 per slide

H&E stained slides – $5.00 per slide

Slide box – $10.00 each

PRICING LIST

Contact us at:

JMD Histology & Histologistics Inc.

151 W Main St Lowr

Dudley, MA 01571

508.461.7207

jcdyson@jmdhistology.com

hans@histologistics.com

 

 

 

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Aqueous Mounting Media’s for Fluorescent and Special Stains, Commercial Brutality?

We do a fair amount of special and fluorescent staining that requires aqueous mounting media and not the toluene based glue.  Normally for special stains I make up a glycerin jelly that has phenol and must be kept in the refrigerator.  This was taught to be the standard of aqueous cover slipping media.  It never dries, sticks to everything and smells terrible.

The fluorescent slides need something different, usually a special fluor mount bought at one of the large chemical companies.  This mount allows for very little light distortion while viewing through a con-focal or fluorescent scope.  The amount of light distortion is called refractive index.  Some of these mounting medias that having a low refractive index of 1.47 can be very expensive, $162.00 plus shipping for 10 ml.

Triple antibody using the confocal microscope.
Triple antibody using the con-focal microscope.

10 ml may be enough to cover slip 300 slides if using only 30 um per slide but that adds $.60 to the cost of every slide.  That may not seem like much but when it’s added to the total after processing, embedding, cutting, antibody plus working hours it can push the average researchers budget to the edge.

I thought, there has to be something better than this.  Large companies get most of their protocols from the old histology books and change 1 micro-gram and it becomes proprietary.  Then the price depends on demand and repeat customers (way too high).  This is what I call commercial brutality.

I’m way to enterprising to pay these prices and be happy about it.  Therefore I too will develop a protocol from the books and change one aspect of it and call it my own.  The difference is, I will formulate it, test it, have others test it, then make it available to all the labs I have daily contact with (10) and help them save money.

One way to stop this money merry go round is to limit profits from unbelievable pricing on medical products.  Paying $500.00 for a water bath that lasts 10 years is absurd!  $162.00 for 10 ml of mounting media is too much!

I have made 25 ml the histo-lo-gis-tics version of aqueous mounting media for about $20.00, but could be 100 ml for $40.00.  That’s the cost if you make it for yourself.  It’s a room temp, permanent media that will last for 2-3 years with anti fading agent and refractive index of 1.46.  So far it’s been tested by 2 independent labs in fluorescent microscopy and passed with no distortion.  2 more labs will be testing it this week and mine soon.

After testing is done, I will update the results, good or not good and post the recipe if it’s useful.

 

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Iron Positive Control Slides Sale $1.37 per slide

Human liver tissue with possible hemochromatosis diagnosis positive control tissues now only $1.37 per slide.  High quality positive control tissues are tested (Prussian blue staining technique) before leaving the building to make sure your complete satisfaction.  The slides will be sectioned to order, so if you have special instructions please send them with your order.  Act now, this sale only last 30 days or when the tissue is all gone.  This sale will be over December 13th 2014.

Human Liver with Iron (Blue) 10x
Human Liver with Iron (Blue) 10x


Chemical fixation of tissue begins at the:
(polls)

 

 

 

 

 

 

 

Contact us at:

Hans B Snyder

Histologistics Inc.

60 Prescott Street

Worcester, MA 01605

hans@histologistics.com

508-308-7800