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The Best Method For Shipping Slides

Our slides are the most important pieces of the project.  Great care is always taken when shipping all slides using a small cell rectangular cut bubble wrap inside the box on top of the slides, then wrap the entire box in bubble wrap a few times around. This gives the safest way possible to eliminate broken slides.  It’s the best skill to teach your interns, cutting bubble wrap, boxing it up and bringing to fed ex, then picking up coffee on the way back! Some slide boxes come with bubble wrap from the vendor, we save those and use when shipping also.

Shipping Slides
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Hematoxylin & Eosin (H&E) Protocol

Safety equipment: Work under a hood with lab coat, gloves, and glasses.

  1. De-paraffin slides in xylene (1) ——————  2 minutes (re-use)
  2. De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
  3. Clear slides in 100% alcohol———————- 2 minutes
  4. Clear slides in 100% alcohol———————- 2 minutes
  5. Hydrate slides in 95% alcohol——————– 2 minutes
  6. Hydrate slides in running water—————–  5 minutes
  7. Stain slides in Harris Hematoxylin————–  10 minutes (re-use)
  8. Rinse slides in running water———————  2 minutes
  9. Differentiate in acid alcohol———————-   1 quick dip (re-use)
  10. Rinse in water————————————–    3 dips
  11. Dip in ammonia water—————————-    15-30 seconds (re-use)
  12. Wash in running tap water———————–    10 minutes
  13. Place in 95% alcohol——————————    2 minutes
  14. Stain in eosin—————————————    1 minute (re-use)
  15. Dehydrate in 95% alcohol————————   1 minute
  16. Dehydrate in 100% alcohol———————-   1 minute
  17. Dehydrate in 100% alcohol———————-   2 minutes
  18. Dehydrate in 100% alcohol———————-   2 minutes
  19. Clear in xylene (3)———————————   2 minutes (re-use)
  20. Clear in xylene (4)———————————   5 minutes (re-use)

Results: Nuclei –Blue   Cytoplasm – Pink     Blood – Red

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Histology Lab

  Histologistics is a full service histology lab. We provide excellent quality histology to clinical facilities, veterinarians and researchers . We can help you with anything.  Please contact us with your histology needs.