Category: FFPE tissues

Human renal cell carcinoma - Vimentin antibody

Vimentin Antibody Validated

by Histology Reference Guide, posted in FFPE tissues, histology service, IHC

We have been working hard to validate many new antibodies for research use.  Vimentin is now available for use on Human, mouse and rat FFPE tissues.

According to the data sheet Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.

Human colon – Vimentin Antibody
Human renal cell carcinoma – Vimentin antibody
Human lung – Vimentin antibody
Human normal kidney – Vimentin antibody
Human colon – Vimentin antibody
Human melanoma – Vimentin antibody
Human Liver – Vimentin antibody
Human skin (melanoma?) H&E

Melanin or Melanoma?

by Histology Reference Guide, posted in FFPE tissues, H&E, Histologistics, Histology, histology service, histopathology, IHC

Melanoma, a heightened subject that should be talked about with, everyone?  How do you tell the difference between melanin and melanoma?  Research facilities are currently exploring this topic and it’s treatment.  But we do know the primary cause, too much UV rays.  The pathologist can see the difference between melanin and melanoma an H&E stained section, can you?.  Here are some examples.

Human Skin H&E with melanin
Human Skin H&E with melanin
Human skin (I think) stained with H&E
Human skin (I think) stained with H&E 40x

For the rest of us, melanoma is better detected by antibody staining (IHC).  There are a few different antibodies, MART1, Melan, HMB but all I have is Granzyme B.

Human skin Granzyme B 10x Possible melanoma
Human skin Granzyme B 40x Possible melanoma

What do you think?

Ovarian Carcinoma

by Histology Reference Guide, posted in FFPE tissues, H&E, Histologistics, Histology, histology service, histopathology

In this tissue, it’s diagnosis is ovarian carcinoma.  It’s stained with H&E and pictures taken at multiple areas.  Some (not posted here) look to have melanin deposits but that will have to be discovered using different staining techniques and will update again here.

Enjoy!

Ovarian Carcinoma H&E