AFIP Lee G. Luna Stain Protocols


One of the most useful staining manuals for histology is the Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology edited by Lee G. Luna 1960.  This contains some of the most used procedures used in histology for the past 50 years.  Most of our stains used for Histologistics are modifications of these protocols.  The book is packed with many of the “old” recipes for making stain solutions by hand using dry and wet chemicals.  For example, there are 7 different hematoxylin recipes that are detailed with side notes about color and ripeness.  In the special stains sections are many modifications to detect the same type of tissues.  There are 6 different protocols to stain for general bacteria., 5 different protocols to stain for acid fast bacteria.  Because this book is no longer in print and has so much useful information, we have decided to copy as much of the book onto this site as possible.  We have already begun, as you see the links above.  The web has tons of histology information on many different websites but not on just one.  When we search the web for histology information, it would be nice to have one site to go to instead of 10-15 sites, each having a little piece of what we need.  This is a long-term project that we hope, will help us and everyone else with all forms of histology.  If you need any help with any phase of histology, post your questions on this site.  If we do not know the solution, we have the resources to find out.  

2 thoughts on “AFIP Lee G. Luna Stain Protocols

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  2. Greetings! I know this is kinda off topic however , I’d figured I’d ask.
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