We recently had a request for the protocol to coat slides with poly-l-lysine. This is good for tissues that fall off using positively charged slides or chrom-alum in the water bath. Sometimes tissues just will not stay on even for just H&E staining. This can be frustrating and bothersome. Frozen sections and sometimes IHC will give these kinds of problems. A histologist will typically try different solutions until finding one that works. The protocol will also be posted in the protocol section for printing.
Poly-L-Lysine
Make your own concentrated solution:
Poly-L-Lysine – 1gm
DH2O – 100ml
Or
Buy pre-made Poly-L-Lysine
1. Dilute stock solution 1ml Poly-L-Lysine to 10ml DH2O
2. Prepare a 50ml working solution in a coplin jar
3. Place plain glass slides (no charge) in the solution for 5 minutes
4. Take slides out and air dry (slides must be completely dry before using)
5. Repeat as necessary
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