In order to study tissue under a microscope, that tissue must be very thin. Most of the tissue to be studied is cut below 15 microns (um). A micron is 1/1000 of a millimeter. Actually 15 microns in histology is considered thick. Routine paraffin and cryosections are cut at 5 microns. This is typically 2-3 cell layers thick. After it is cut, the sections are placed on top of a glass microscope slide. The slide usually has some kind of adhesive to keep the tissue from falling off. The slide is then dried in a 60C oven or left overnight. This ensures no water will be on the slide or tissue. It is then dipped in a series of chemicals to stain the tissue and make it permanent for later viewing under a microscope. It is this final product that is considered a histology slide.
An expert Histology Service