This elastic stain is far easier than Verhoeff Van Gieson stain but the chemical can be more unusual to find.

Run a control slide.             Control Tissue – Aorta, Skin

Safety equipment: Work under a hood with lab coat, gloves, and glasses.

1. De-paraffin slides in xylene (1) —————– 2 minutes (re-use)
2. De-paraffin slides in xylene (2)—————— 2 minutes (re-use)
3. Clear slides in 100% alcohol——————— 2 minutes
4. Clear slides in 100% alcohol——————— 2 minutes
5. Hydrate slides in 95% alcohol——————- 2 minutes
6. Hydrate slides in running water—————– 5 minutes
7. Stain in Orcein ————————————– 30 minutes (re-use)
8. 70% alcohol —————————————– 10 minutes
9. 70% alcohol —————————————– 10 minutes
10. 10.  70% alcohol —————————————- 10 minutes
11. 11.  Rinse in running water ————————– 1 minute
12. 12.  Stain in Harris Hematoxylin ——————– 1 minute (re-use)
13. 13.  Rinse in running water ————————– 1 minute
14. 14.  Acid alcohol —————————————- 1 dip (re-use)
15. 15.  Rinse in running water ————————– 5 minutes
16. 16.  Dehydrate in 95% alcohol———————- 30 seconds
17. 17.   Dehydrate in 100% alcohol——————- 1 minute
18. 18.   Dehydrate in 100% alcohol——————- 2 minutes
19. 19.   Dehydrate in 100% alcohol——————- 2 minutes
20. 20.   Clear in xylene (3)—————————— 2 minutes (re-use)
21. 21.   Clear in xylene (4)—————————— 5 minutes (re-use)

Results:         Elastic Fibers – Dark Pink

 

 

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