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This elastic stain is far easier than Verhoeff Van Gieson stain but the chemical can be more unusual to find.
Run a control slide. Control Tissue – Aorta, Skin
Safety equipment: Work under a hood with lab coat, gloves, and glasses.
- De-paraffin slides in xylene (1) —————– 2 minutes (re-use)
- De-paraffin slides in xylene (2)—————— 2 minutes (re-use)
- Clear slides in 100% alcohol——————— 2 minutes
- Clear slides in 100% alcohol——————— 2 minutes
- Hydrate slides in 95% alcohol——————- 2 minutes
- Hydrate slides in running water—————– 5 minutes
- Stain in Orcein ————————————– 30 minutes (re-use)
- 70% alcohol —————————————– 10 minutes
- 70% alcohol —————————————– 10 minutes
- 10. 70% alcohol —————————————- 10 minutes
- 11. Rinse in running water ————————– 1 minute
- 12. Stain in Harris Hematoxylin ——————– 1 minute (re-use)
- 13. Rinse in running water ————————– 1 minute
- 14. Acid alcohol —————————————- 1 dip (re-use)
- 15. Rinse in running water ————————– 5 minutes
- 16. Dehydrate in 95% alcohol———————- 30 seconds
- 17. Dehydrate in 100% alcohol——————- 1 minute
- 18. Dehydrate in 100% alcohol——————- 2 minutes
- 19. Dehydrate in 100% alcohol——————- 2 minutes
- 20. Clear in xylene (3)—————————— 2 minutes (re-use)
- 21. Clear in xylene (4)—————————— 5 minutes (re-use)
Results: Elastic Fibers – Dark Pink
An Expert Histology Service