Movat’s Modified Pentachrome Protocol


This is a quick version of Movat’s Pentachrome.  The long version is an overnight stain, I hate waiting till the next day to find out if the stain worked.  It is sometimes hard to visualize all 5 colors but with a aorta in the heart, it is possible.  The stain picture will have to be edited in later.

Control tissue that I use: Skin, Aorta in the heart

Safety equipment: Work under a hood with lab coat, gloves, and glasses.

  1. Deparaffinize and hydrate to DH2O
  2. Preheat Alkaline alcohol in 60C oven or water bath
  3. Mordant in bouin’s at 60C water bath——- 30 minutes (under hood!) (re-use)
  4. Cool—————————————– 10 minutes in room temp water
  5. Wash in running water—————– 10 minutes
  6. 1% Alcian blue————————— 25 minutes (re-use)
  7. Wash in running water—————– 5 minutes
  8. Alkaline alcohol at 60C —————- 10 minutes
  9. Wash in running water—————— 5 minutes
  10. Weigerts———————————– 15 minutes (dump)
  11. Differentiate in 2% ferric chloride for- 3 minutes (re-use)
  12. Rinse in H2O——————————- 10 seconds
  13. Sodium thiosulfate for——————– 1 minute (re-use)
  14. Wash in running water——————- 5 minutes
  15. Crocein scarlet/ Acid fuchsin———- 1.5 minutes (re-use)
  16. Wash in running water—————— 5 minutes
  17. 5% Phosphotungstic acid————– 5 minutes (re-use)
  18. Transfer directly to 1% acetic acid–  5 minutes (re-use)
  19. Wash in running water—————— 5 minutes
  20. Dehydrate quickly 95% alc. 1 min/2x    100% alc. 1 min
  21. Alcoholic saffron————————– 60 minutes (re-use)
  22. Rinse in 2 changes of 100% alcohol, 1 min each
  23. Clear in xylene and mount


Nuclei and elastic fibers—Black


Ground substance and mucin–Blue

Fibrinoid, fibrin–Intense Red



An Expert Histology Service

42 thoughts on “Movat’s Modified Pentachrome Protocol

  1. I have to thank you for the efforts you’ve put in penning this site.

    I am hoping to check out the same high-grade content by you later on as
    well. In fact, your creative writing abilities has motivated me
    to get my own, personal website now 😉

  2. Hmm is anyone else having problems with the images on this blog loading?

    I’m trying to figure out if its a problem
    on my end or if it’s the blog. Any suggestions would be greatly appreciated.

  3. Its such as you read my mind! You appear to understand so
    much approximately this, such as you wrote the guide in it or something.
    I feel that you simply could do with some percent to pressure the
    message home a bit, but instead of that, that is magnificent blog.
    A great read. I’ll certainly be back.

  4. fantastic pоѕt, very informativе. I ponder why
    the other speϲialists of this ѕector do not undеrstand this.
    You must proceed your writing. I am surе, ƴou’ve a huge rеaders’ base already!

  5. Way cool! Some very valid points! I appreciate you penning this article plus the rest of the website is really good.

  6. Most people who go into business do so because they’re somewhat of an expert
    in their niche. Bloggers and site owners that sell advertising space will love
    it because they will experience higher click through rates
    and higher returns. Communicate with your affiliate marketers

  7. Article Source: @ Soomla, Entrepreneurs x 3, Blogger x 4 In my current position as CEO of SOOMLA my focus is on mobile game monetization. So before we discuss some of
    the proven ways to plan an effective mobile app development business, let’s first take a look the history behind all of this.
    With too many questions and confusion, many people tend to download the games with highest number of reviews,
    ratings and download, whether they like it or not.

  8. Excellent blog here! Additionally your web site so much up
    very fast! What web host are you the usage of?
    Can I get your affiliate hyperlink to your host?
    I want my web site loaded up as fast as yours lol

  9. Wow, that’s what I was exploring for, what a data! existing here at
    this web site, thanks admin of this web page.

  10. Thanks for another excellent article. Where else may just anyone get that kind of information in such
    an ideal way of writing? I’ve a presentation subsequent
    week, and I’m at the look for such information.

  11. I know thhis web page offers quality dependent articles and additional information, is there aany other site
    which gives such stuff in quality?

  12. An impressive share! I have just forwarded this onto a co-worker who was doing a little homework on this.
    And he in fact bought me breakfast simply because I discovered it for him…
    lol. So let me reword this…. Thank YOU for the
    meal!! But yeah, thanks for spending time to talk about this subject here on your website.

  13. Hey there! Do you use Twitter? I’d like to follow you if
    that would be ok. I’m undoubtedly enjoying your blog and look forward to
    new updates.

  14. You are so cool! I don’t suppose I’ve read
    a single thing like this before. So wonderful to
    discover someone with genuine thoughts on this topic. Seriously..
    many thanks for starting this up. This web site is one thing that is needed on the internet,
    someone with a bit of originality!

  15. Pretty nice post. I just stumbled upon your blog and wanted to mention that I’ve really loved browsing
    your blog posts. In any case I will be subscribing in your
    rss feed and I hope you write again very soon!

  16. I blog frequently and I seriously thank you for your information.
    This great article has really peaked my interest.
    I’m going to book mark your site and keep checking for new details about once
    per week. I opted in for your RSS feed as well.

  17. Great items from you, man. I have take note your stuff previous to and you are simply too fantastic.
    I actually like what you’ve obtained right here, certainly like what
    you’re stating and the way wherein you say it.

    You’re making it entertaining and you still take care of to stay it sensible.
    I can’t wait to read far more from you. This is really a great web site.

  18. You could certainly see your enthusiasm in the article you write.

    The world hopes for more passionate writers like you who are not afraid to say how they believe.
    At all times go after your heart.

  19. Aw, thіs was a гeally nicce post. Finding tɦe time and actual effort
    tօ creɑtᥱ a superb article… Ьut what can I ѕay… I hesiitate
    а wҺole lot ɑnd don’t seеm to ɡet neaгly anything dоne.

  20. Hi,
    Great article! I am relatively new to Histology and I was wondering if this stain can be de-stained and repeated if the slides didn’t stain well? I tried the Masson de-stain protocol and it worked great! thanks!

  21. This website really has all the information and facts I wanted concerning this subject and
    didn’t know who to ask.

Leave a ReplyCancel reply