Safety equipment: Work under a hood with lab coat, gloves, and glasses.

1. De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
2. De-paraffin slides in xylene (2)—————— 2 minutes (re-use)
3. Clear slides in 100% alcohol——————— 2 minutes
4. Clear slides in 100% alcohol——————— 2 minutes
5. Hydrate slides in 95% alcohol——————- 2 minutes
6. Hydrate slides in running water—————– 5 minutes
7. 0.5% periodic acid ——————————– 5 minutes (re-use)
8. Schiff’s reagent at 37C—————————- 15 minutes (re-use)
9. Wash in running water ————————— 10 minutes
10. If staining for glycogen, Hematoxylin ——— 20 seconds (re-use)
11. If staining for fungus, light green ————— 30 seconds (re-use)
12. Dehydrate in 95% alcohol———————— 30 seconds
13. Dehydrate in 100% alcohol———————- 1 minute
14. Dehydrate in 100% alcohol———————- 2 minutes
15. Dehydrate in 100% alcohol———————- 2 minutes
16. Clear in xylene (3)——————————— 2 minutes (re-use)
17. Clear in xylene (4)——————————— 5 minutes (re-use)

Results:           Hematoxylin – Glycogen, mucin, fibrin, or thrombi, colloid droplets, hyaline of arteriosclerosis, hyaline deposits in glomeruli, granular cells in the renal arterioles where preserved, most basement membranes, colloid of pituitary stalks and thyroid, amyloid infiltration may show a positive reaction – Rose to Purplish Red

                        Nuclei – Blue

Results:          Light Green – Fungi – Red                 Background – Pale Green

 

 

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