Special stains can be finicky. If you do one little thing wrong, it might not come out the way you want. We have seen so many different problems in a variety of settings that it pays to be precise in all calculations with regard to solutions creations and protocol times. For example, A stain might look abnormal if left in running water too long. The stain may look pale or devoid of a background color. Stains can be re-done by washing out with copious amounts of water. Sometimes this can be achieved if left in alcohol other than 100%. Water washes the stain out. It can leave bare traces of what there once was.
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