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Run a control slide. Control tissue – Tissue with amyloid
Safety equipment: Work under a hood with lab coat, gloves, and glasses.
- De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
- De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
- Clear slides in 100% alcohol———————- 2 minutes
- Clear slides in 100% alcohol———————- 2 minutes
- Hydrate slides in 95% alcohol——————– 2 minutes
- Hydrate slides in running water —————– 5 minutes
- 1% Congo red ————————————— 60 minutes (re-use)
- Rinse in water ————————————— 1 minute
- Alkaline Alcohol ————————————- until background clear (re-use)
- Wash in running water —————————- 5 minutes
- Harris Hematoxylin ——————————— 30 seconds (re-use)
- Wash in running water —————————- 10 minutes
- Dehydrate in 95% alcohol———————— 30 seconds
- Dehydrate in 100% alcohol——————— 1 minute
- Dehydrate in 100% alcohol——————— 2 minutes
- Dehydrate in 100% alcohol——————— 2 minutes
- Clear in xylene (3)——————————– 2 minutes (re-use)
- Clear in xylene (4)——————————– 5 minutes (re-use)
- Coverslip
Results: Amyloid – Pink to red Nuclei – Blue
With polarizing microscope
Amyloid – Green Birefringence
An Expert Histology Service
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