Run a control slide.                                     Control tissue – Tissue with amyloid

Safety equipment: Work under a hood with lab coat, gloves, and glasses.

1. De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
2. De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
3. Clear slides in 100% alcohol———————- 2 minutes
4. Clear slides in 100% alcohol———————- 2 minutes
5. Hydrate slides in 95% alcohol——————– 2 minutes
6. Hydrate slides in running water —————– 5 minutes
7. 1% Congo red ————————————— 60 minutes (re-use)
8. Rinse in water ————————————— 1 minute
9. Alkaline Alcohol ————————————- until background clear (re-use)
10. Wash in running water —————————- 5 minutes
11. Harris Hematoxylin ——————————— 30 seconds (re-use)
12. Wash in running water —————————- 10 minutes
13. Dehydrate in 95% alcohol———————— 30 seconds
14. Dehydrate in 100% alcohol——————— 1 minute
15. Dehydrate in 100% alcohol——————— 2 minutes
16. Dehydrate in 100% alcohol——————— 2 minutes
17. Clear in xylene (3)——————————– 2 minutes (re-use)
18. Clear in xylene (4)——————————– 5 minutes (re-use)
19. Coverslip

Results:         Amyloid – Pink to red                     Nuclei – Blue

With polarizing microscope

Amyloid – Green Birefringence

An Expert Histology Service