Safety equipment: Work under a hood with lab coat, gloves, and glasses.

1. De-paraffin slides in xylene (1) ——————  2 minutes (re-use)
2. De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
3. Clear slides in 100% alcohol———————- 2 minutes
4. Clear slides in 100% alcohol———————- 2 minutes
5. Hydrate slides in 95% alcohol——————– 2 minutes
6. Hydrate slides in running water—————–  5 minutes
7. Stain slides in Harris Hematoxylin————–  10 minutes (re-use)
8. Rinse slides in running water———————  2 minutes
9. Differentiate in acid alcohol———————-   1 quick dip (re-use)
10. Rinse in water————————————–    3 dips
11. Dip in ammonia water—————————-    15-30 seconds (re-use)
12. Wash in running tap water———————–    10 minutes
13. Place in 95% alcohol——————————    2 minutes
14. Stain in eosin—————————————    1 minute (re-use)
15. Dehydrate in 95% alcohol————————   1 minute
16. Dehydrate in 100% alcohol———————-   1 minute
17. Dehydrate in 100% alcohol———————-   2 minutes
18. Dehydrate in 100% alcohol———————-   2 minutes
19. Clear in xylene (3)———————————   2 minutes (re-use)
20. Clear in xylene (4)———————————   5 minutes (re-use)

Results: Nuclei –Blue   Cytoplasm – Pink     Blood – Red