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Safety equipment: Work under a hood with lab coat, gloves, and glasses.
- De-paraffin slides in xylene (1) —————— 2 minutes (re-use)
- De-paraffin slides in xylene (2)——————- 2 minutes (re-use)
- Clear slides in 100% alcohol———————- 2 minutes
- Clear slides in 100% alcohol———————- 2 minutes
- Hydrate slides in 95% alcohol——————– 2 minutes
- Hydrate slides in running water—————– 5 minutes
- Stain slides in Harris Hematoxylin————– 10 minutes (re-use)
- Rinse slides in running water——————— 2 minutes
- Differentiate in acid alcohol———————- 1 quick dip (re-use)
- Rinse in water————————————– 3 dips
- Dip in ammonia water—————————- 15-30 seconds (re-use)
- Wash in running tap water———————– 10 minutes
- Place in 95% alcohol—————————— 2 minutes
- Stain in eosin————————————— 1 minute (re-use)
- Dehydrate in 95% alcohol———————— 1 minute
- Dehydrate in 100% alcohol———————- 1 minute
- Dehydrate in 100% alcohol———————- 2 minutes
- Dehydrate in 100% alcohol———————- 2 minutes
- Clear in xylene (3)——————————— 2 minutes (re-use)
- Clear in xylene (4)——————————— 5 minutes (re-use)
Results: Nuclei –Blue Cytoplasm – Pink Blood – Red