Hello everyone, we are proud to announce that we have been working with Woodland Pharmaceuticals in their project to identify a liver to lung metastasis model (MHCC97H) and are evaluating the lung cancer SQ A549 for lung lesions as well. At this point Woodland Pharmaceuticals is establishing a stable luciferase to express tumor cell lines with their partner SBH Sciences so metastasis can be quantified. They have positive control compounds that will mechanistically block most/all of this metastasis. We have done all their histology so far and have much more to do. Follow us with this exciting project.
We did this as a test to see different versions of luxol fast blue (LFB). LFB is a stain for the myelin sheath or myelinated axons in brain and spinal cord. We tested on rat brain. There are 4 variations. They are; LFB / Cresyl violet (CR), LFB /H&E, LFB / Periodic acid schiff (PAS) and LFB / nuclear fast red (NFR). The H&E and NFR versions are for background staining only. The PAS could show glycogen or fungus present and the CR shows nissl bodies.
We specialize in research projects. All projects received are completed by the person who receives it. This eliminates problems created by incomplete or incorrect communication. On small projects all phases of histology can usually be completed within 2 days of receipt. During the first day, we log, gross and cassette if necessary, and process. On the second day, we embed, cut and stain. To make sure everything is correct, we check every slide against its block and manifest 3 times by someone other than the person who cut them. Doing this takes a little longer but eliminates most technical errors. For this reason we require a manifest of all specimens sent before any work is done. manifest-list-sheet.xlsx